Myofibrillar differences among mammalian skeletal muscle fibres at the ultrastructural level. A comparison of immunocytochemical and morphometrical parameters.
Myofibrillar differences among mammalian skeletal muscle fibres at the ultrastructural level. A comparison of immunocytochemical and morphometrical parameters.
复制标题
哺乳动物骨骼肌纤维在超微结构水平上的肌原纤维差异。
DOI：
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发表时间：
1980
影响因子：
6.6
通讯作者：
W. van Raamsdonk
中科院分区：
文献类型：
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作者：
G. te Kronnie;C. Pool;G. Scholten;W. van Raamsdonk
在光镜下，两种类型（I， II）的骨骼肌纤维可以区分与肌球蛋白同工酶抗体。在超微结构水平上，z线宽度的差异导致了肌纤维的分类。在这项研究中，我们使用免疫铁蛋白和抗肌球蛋白同工酶血清进行超切片，在超微结构水平上区分成年小鼠比目鱼和植物的I型和II型纤维。足底鱼的肌纤维分为II型纤维，比目鱼的肌纤维分为I型和II型纤维。在免疫鉴定的纤维中，测量了丝在z线上的重叠。plantaris的II型纤维具有窄的z线，而M. soleus的I型和II型纤维具有宽的z线。我们得出结论，基于z线宽度的纤维分类不同于I型/ II型分类。抗肌球蛋白抗体只与a带反应。在连续切片中，肌凝蛋白同工酶可以明确地识别。这是进一步研究肌球蛋白同工酶在“混合”肌纤维中的分布的先决条件。
In the light microscope two types (I, II) of skeletal muscle fibres can be distinguished with antibodies against myosin isozymes. At the ultrastructural level a difference in Z-line width has led to muscle fibre classification. In this study we distinguish at the ultrastructural level between type I and type II fibres of the M. soleus and M. plantaris of adult mice using ultracryosections with immuno-ferritin and antisera against myosin isozymes. Muscle fibres of the M. plantaris are identified as type II fibres and the fibres of the M. soleus are divided in type I and type II fibres. In the immunologically identified fibres the filament overlap in the Z-line was measured. The type II fibres of the M. plantaris have narrow Z-lines, whereas type I and type II fibres of the M. soleus have wide Z-lines. We conclude that a classification of fibres based on Z-line width differs from the type I/type II classification. The antimyosin antibodies react exclusively with the A-band. In serial sections the myosin isozymes can be identified unambiguously. This is a prerequisite for further studies of myosin isozyme distribution in "mixed" muscle fibres.