TiO2 with Confined Water Boosts Ultrahigh Selective Enrichment of Phosphorylated Proteins.
TiO2 with Confined Water Boosts Ultrahigh Selective Enrichment of Phosphorylated Proteins.
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DOI：
10.1021/acsami.2c03158
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发表时间：
2022-04
影响因子：
9.5
通讯作者：
Xiaotao Yang;Xiaofei Zhang;Yulong Li;Xiuling Li;Xinmiao Liang;Ye Tian;Lei Jiang
中科院分区：
文献类型：
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作者：
Xiaotao Yang;Xiaofei Zhang;Yulong Li;Xiuling Li;Xinmiao Liang;Ye Tian;Lei Jiang
在生物样品中磷酸化蛋白的选择性浓缩过程中，由于疏水作用，非磷酸化蛋白(NPPs)吸附在富集剂上，导致目标PPS选择性差，回收率低。在这里，我们制备了超亲水的二氧化钛包覆的多孔二氧化硅微球，并提高了对添加了2000质量倍的NPP干扰的标准PP的显著选择性。超亲水微球的优异性能归功于二氧化钛与聚苯硫醚之间的配位作用，通过超亲水形成的承压水层使核聚糖核酮内部疏水性区域保持在紧凑的结构中，从而避免了对核聚糖核酸的不可逆吸附，这一点通过单分子力谱、圆二色谱和石英晶体微天平进行了验证。这一浓缩策略有望解决蛋白质组学中的挑战，并揭示生物分子与超润湿性之间的相互作用。
In the selective enrichment of phosphorylated proteins (PPs) from biological samples, the non-phosphorylated proteins (NPPs) adhered onto enrichment adsorbents due to the hydrophobic interaction, resulting in poor selectivity and low recovery of target PPs. Herein, superhydrophilic TiO2-coated porous SiO2 microspheres are prepared and boost remarkable selectivity toward standard PP spiked with 2000 mass-fold NPP interference. The outstanding performance of the superhydrophilic microspheres is attributed to the coordination interaction between TiO2 and PPs, and the confined water layer generated from superhydrophilicity avoids the irreversible adsorption of NPPs by keeping NPP inner hydrophobic regions in a compact structure, which is verified by single molecule force spectroscopy, circular dichroism, and quartz crystal microbalance. This strategy for enrichment is expected to solve the challenge in proteomics and sheds light on the interactions between biomolecules and superwettability.