Direct contacts with colon cancer cells regulate the differentiation of bone marrow mesenchymal stem cells into tumor associated fibroblasts
Direct contacts with colon cancer cells regulate the differentiation of bone marrow mesenchymal stem cells into tumor associated fibroblasts
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与结肠癌细胞的直接接触调节骨髓间充质干细胞向肿瘤相关成纤维细胞的分化
DOI：
10.1016/j.bbrc.2014.07.074
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发表时间：
2014-08-15
影响因子：
3.1
通讯作者：
Li, Zhuoyu
中科院分区：
文献类型：
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作者：
Peng, Yanan;Li, Zongwei;Li, Zhuoyu
肿瘤-间质相互作用被称为肿瘤进展中的基本事件。骨髓间充质干细胞（BMSCs）可迁移至肿瘤间质，分化为肿瘤相关成纤维细胞（TAF）样细胞，这是一种主要的促肿瘤基质细胞。然而，很少有人确切地知道这种转变的贡献者。在这里，使用结肠癌细胞和BMSCs的体外直接共培养模型，我们确定结肠癌细胞可以诱导相邻的BMSCs表现出TAFs的典型特征，增加α-平滑肌肌动蛋白（α-SMA）的表达。重要的是，本数据还揭示了活化的Notch信号传导通过下游TGF-β/Smad信号传导途径介导BMSC向TAF的转化。(C)2014爱思唯尔公司All rights reserved.
Tumor-stroma interactions are referred to as essential events in tumor progression. There has been growing attention that bone marrow-derived mesenchymal stem cells (BMSCs) can travel to tumor stroma, where they differentiate into tumor-associated fibroblast (TAF)-like cells, a predominant tumor-promoting stromal cell. However, little is definitively known about the contributors for this transition. Here, using an in vitro direct co-culture model of colon cancer cells and BMSCs, we identify that colon cancer cells can induce adjoining BMSCs to exhibit the typical characteristic of TAFs, with increased expression of alpha-smooth muscle actin (alpha-SMA). Importantly, the present data also reveals that activated Notch signaling mediates transformation of BMSCs to TAFs through the downstream TGF-beta/Smad signaling pathway. (C) 2014 Elsevier Inc. All rights reserved.