MONOCLONAL ANTIBODY-DEFINED ANTIGEN OF HUMAN UTERINE ENDOMETRIAL CARCINOMAS IS LEB
MONOCLONAL ANTIBODY-DEFINED ANTIGEN OF HUMAN UTERINE ENDOMETRIAL CARCINOMAS IS LEB
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DOI：
10.1093/oxfordjournals.jbchem.a122734
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发表时间：
1989-05-01
影响因子：
2.7
通讯作者：
NAGAI, Y
中科院分区：
文献类型：
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作者：
IWAMORI, M;SAKAYORI, M;NAGAI, Y
通过用人子宫内膜腺癌细胞系SNG-II免疫小鼠产生的单克隆抗体MSN-1与来自子宫内膜腺癌患者的癌组织的中性鞘糖脂具有强烈的特异性反应。鞘糖脂抗原从合并的人胎粪中纯化，其含有浓度为1.95 μ mol/g干重的抗原。经NMR、负离子FABMS、全甲基化分析和单克隆抗Lc 4 Cer抗体的TLC免疫染色确定其结构，并推断其为人刘易斯血液系统的III 4 IV 2 Fuc 2 Lc 4 Cer，Leb抗原。在ELISA上，发现单克隆抗体与Leb强烈反应，与莱亚轻微反应，与A、B、H或IV 2 FucGg 4 Cer完全不反应。Lea-b+血型患者的癌性区域中Leb的量与相同患者的正常区域中的Leb的量相比显著增加，并且它是莱亚+B-血型患者的癌性组织中新出现的抗原。
A monoclonal antibody, MSN-1, generated by immunizing a mouse with a human uterine endometrial adenocarcinoma cell line, SNG-II, was strongly and specifically reactive with neutral glycosphingolipids from cancer tissues of patients with uterine endometrial adenocarcinomas. The glycosphingolipid antigen was purified from pooled human meconia, which contained the antigen at the concentration of 1.95 .mu.mol/g dry weight. Its structure was determined by NMR, negative ion FABMS, permethylation analysis, and TLC-immunostaining with monoclonal anti-Lc4Cer antibody, and was concluded to be the III4IV2Fuc2Lc4Cer,Leb antigen of the human Lewis blood system. On ELISA, the monoclonal antibody was found to be strongly reactive with Leb, slightly with Lea and not at all with A, B, H, or IV2FucGg4 Cer. The amount of Leb in cancerous regions in the patients with the Lea-b+ blood group was significantly increased compared to that in normal regions in the same patients, and it was a newly appearing antigen in the cancerous tissue of a patient with the Lea+b- blood group.