Expression profiles of 507 proteins from a biotin label- based antibody array in human colorectal cancer
Expression profiles of 507 proteins from a biotin label- based antibody array in human colorectal cancer
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DOI：
10.3892/or.2013.2935
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发表时间：
2014-03-01
期刊：
影响因子：
4.2
通讯作者：
Masaki, Tsutomu
中科院分区：
文献类型：
--
作者：
Miyoshi, Hisaaki;Morishita, Asahiro;Masaki, Tsutomu
分子靶向治疗是晚期结直肠癌（CRC）患者最有希望的治疗方法之一。然而，多种蛋白质在 CRC 中的表达水平未知。本研究的目的是确定与结直肠癌发生和癌症发展相关的各种蛋白质的表达水平。我们使用基于生物素标记的抗体阵列检测了 6 个人类 CRC 组织中 507 种靶蛋白的表达水平。我们还分析了 CRC 患者的临床病理特征。在结直肠癌组织中，IL-1、GRO、Glut5、MIG、ICAM-5、VE-钙粘蛋白、uPA 和瘦素 R 与正常结肠组织中的水平相比有所增加。与非直肠癌中的水平相比，直肠癌中 MPIF-1/CCL23、FGF R5、MIP2、SAA 和 IL-18 R 的表达强烈上调。这些数据表明差异蛋白表达谱在不同条件下存在，包括癌发生和结直肠癌定位。因此，使用基于生物素标记的抗体蛋白阵列对蛋白表达水平进行详尽的分析是为结直肠癌患者确定新的个体疗法的潜在有用工具。
Molecular-targeted therapy is one of the most promising therapies for patients with advanced-stage colorectal cancer (CRC). However, a wide range of proteins have unknown expression levels in CRC. The purpose of the present study was to determine the expression levels of various proteins related to colorectal carcinogenesis and cancer development. We examined the expression levels of 507 target proteins using a biotin label-based antibody array in 6 human CRC tissues. We also analyzed the clinicopathological features of CRC patients. In CRC tissues, IL-1, GRO, Glut5, MIG, ICAM-5, VE-cadherin, uPA and Leptin R were increased when compared to levels in normal colon tissues. MPIF-1/CCL23, FGF R5, MIP2, SAA and IL-18 R were strongly upregulated in rectal cancer when compared to the levels in non-rectal cancer. These data suggest that differential protein expression profiles exist under different conditions, including carcinogenesis and CRC localization. Therefore, an exhaustive analysis of protein expression levels using a biotin label-based antibody protein array is a potentially useful tool for identifying novel individual therapies for CRC patients.