Relationship Between Putative eps Genes and Production of Exopolysaccharide in Lactobacillus casei LC2W.
Relationship Between Putative eps Genes and Production of Exopolysaccharide in Lactobacillus casei LC2W.
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DOI：
10.3389/fmicb.2018.01882
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发表时间：
2018
影响因子：
5.2
通讯作者：
Ai L
中科院分区：
文献类型：
--
作者：
Song X;Xiong Z;Kong L;Wang G;Ai L
干酪乳杆菌LC 2 W是一株能产生具有降血压活性的胞外多糖的益生菌。由于EPS生物合成的调控机制尚不清楚，本研究对LC 2 W中eps基因与EPS合成的关系进行了研究。对EPS生物合成基因簇中的几个相关基因进行了缺失、过表达和互补。结果表明，葡萄糖-1-磷酸胸苷基转移酶基因（LC2W_2179）、EPS生物合成蛋白（LC2W_2188）和EPS生物合成蛋白（LC2W_2189）与EPS生物合成有关。当这三个基因缺失时，EPS滴度分别下降15%、13%和21%。当它们过表达时，EPS滴度增加16%、10%和18%。当它们互补时，EPS滴度与野生型菌株相似。这项工作表明LC 2 W中的三个eps基因在EPS的产生中发挥着重要作用。
Lactobacillus casei LC2W, a probiotic strain, can produce exopolysaccharide (EPS) with anti-hypertensive bioactivity. The relationship between eps genes and EPS synthesis in LC2W due to unclear regulation mechanism of EPS biosynthesis was investigated. The several relevant genes in EPS biosynthetic gene cluster were deleted, overexpressed and complemented. The results suggested that glucose-1-phosphate thymidyltranseferase gene (LC2W_2179), uncharacterized EPS biosynthesis protein (LC2W_2188), and EPS biosynthesis protein (LC2W_2189) were related to EPS biosynthesis. EPS titer decreased 15, 13, and 21% when the three genes were deleted, respectively. When they were overexpressed, EPS titer increased 16, 10, and 18%. When they were complemented, EPS titer was similar to the wild-type strain. This work showed the three eps genes from LC2W played important roles on EPS production.
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作者：
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