Eosinophil-granule major basic protein, a C-type lectin, binds heparin.
Eosinophil-granule major basic protein, a C-type lectin, binds heparin.
复制标题
嗜酸性粒细胞颗粒主要碱性蛋白（一种 C 型凝集素）与肝素结合。
DOI：
10.1021/bi051112b
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发表时间：
2005
期刊：
影响因子：
2.9
通讯作者：
Acharya,KRavi
中科院分区：
文献类型：
--
作者：
Swaminathan,GJawahar;Myszka,DavidG;Katsamba,PhinikoulaS;Ohnuki,LyoE;Gleich,GeraldJ;Acharya,KRavi
嗜酸性粒细胞主要碱性蛋白（EMBP）是嗜酸性粒细胞次级颗粒的一种成分，与细胞毒性和过敏性疾病（如哮喘）的介导有关。它是C型凝集素家族的一员，但缺乏在该家族其他成员中所见的Ca 2+和碳水化合物结合位点。在这里，我们报告了在不存在Ca 2+的情况下与肝素双糖复合的EMBP的晶体结构，这是第一次报告任何含有这种糖的C-凝集素。我们还提供了直接的证据结合EMBP肝素和肝素二糖的表面等离子体共振。我们认为EMBP识别的糖很可能是肝素等蛋白聚糖，从而对EMBP的功能进行了新的解释。
The eosinophil major basic protein (EMBP), a constituent of the eosinophil secondary granule, is implicated in cytotoxicity and mediation of allergic disorders such as asthma. It is a member of the C-type lectin family, but lacks a Ca2+- and carbohydrate-binding site as seen in other members of this family. Here, we report the crystal structure of EMBP in complex with a heparin disaccharide and in the absence of Ca2+, the first such report of any C-lectin with this sugar. We also provide direct evidence of binding of EMBP to heparin and heparin disaccharide by surface plasmon resonance. We propose that the sugars recognized by EMBP are likely to be proteoglycans such as heparin, leading to new interpretations for EMBP function.