Molecular analysis of the bacterial microbiota in the human stomach
Molecular analysis of the bacterial microbiota in the human stomach
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DOI：
10.1073/pnas.0506655103
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发表时间：
2006-01-17
影响因子：
11.1
通讯作者：
Relman, DA
中科院分区：
文献类型：
--
作者：
Bik, EM;Eckburg, PB;Relman, DA
人类胃的微生物群和幽门螺杆菌定植对其组成的影响在很大程度上仍然未知。我们通过使用小亚基16 S rDNA克隆文库方法表征了人类胃粘膜内的细菌多样性，并分析了23份胃镜活检样本中通过宽范围细菌PCR产生的1，833个序列。一个多样化的社区128个不同类型的被确定，具有多样性在这个网站比以前描述的。大部分序列归属于变形菌门、厚壁菌门、放线菌门、拟杆菌门和梭杆菌门。其中10%的细菌类型以前没有被鉴定过，包括一种与异常球菌有关的生物，这种生物的亲戚在极端环境中被发现，但以前在人类中没有报道过。19名受试者的胃克隆文库中含有H. pylori rDNA;然而，这些受试者中只有12人的H. pylori的常规实验室方法。统计分析显示，胃生态系统存在很大程度的个体间变异性。H.幽门不影响胃群落的组成。这一胃细菌rDNA数据集与其他研究中描述的人类口腔和食管的序列集显著不同，表明人类胃可能是一个独特的微生物生态系统的家园。胃微生物群可能在人类健康和疾病中发挥重要的，尚未发现的作用。
The microbiota of the human stomach and the influence of Helicobacter pylori colonization on its composition remain largely unknown. We characterized bacterial diversity within the human gastric mucosa by using a small subunit 16S rDNA clone library approach and analyzed 1,833 sequences generated by broad-range bacterial PCR from 23 gastric endoscopic biopsy samples. A diverse community of 128 phylotypes was identified, featuring diversity at this site greater than previously described. The majority of sequences were assigned to the Proteobacteria, Firmicutes, Actinobacteria, Bacteroidetes, and Fusobacteria phyla. Ten percent of the phylotypes were previously uncharacterized, including a Deinococcus-related organism, relatives of which have been found in extreme environments but not reported before in humans. The gastric clone libraries from 19 subjects contained H. pylori rDNA; however, only 12 of these subjects tested positive for H. pylori by conventional laboratory methods. Statistical analysis revealed a large degree of intersubject variability of the gastric ecosystem. The presence of H. pylori did not affect the composition of the gastric community. This gastric bacterial rDNA data set was significantly different from sequence collections of the human mouth and esophagus described in other studies, indicating that the human stomach may be home to a distinct microbial ecosystem. The gastric microbiota may play important, as-yet-undiscovered roles in human health and disease.