Exposure to aflatoxin B1 in animal-feed production plant workers.
Exposure to aflatoxin B1 in animal-feed production plant workers.
复制标题
DOI：
10.1289/ehp.9399195
复制
发表时间：
1993-03
影响因子：
10.4
通讯作者：
Autrup H
中科院分区：
文献类型：
--
作者：
Autrup JL;Schmidt J;Autrup H
采用黄曲霉毒素B1（AFB）与血清白蛋白结合的方法，对动物饲料加工厂中AFB的暴露进行了评估。用链霉蛋白酶消化白蛋白级分，并在通过ELISA定量之前在C18 Sepak柱和aflatest亲和柱上纯化消化物。可检测性水平为5 pg/mg白蛋白。这些工人作为自己的对照组，在休假返回和工作4周后采集血样。45个样本中共有7个样本呈AFB阳性，估计平均每日摄入量为64 ng AFB/kg体重。暴露的工人曾下过被黄曲霉毒素B污染的货物，或在粉尘中含有可检测到的黄曲霉毒素B的地方工作。暴露工人的血清对黄曲霉毒素B1-表位比未暴露的丹麦对照组有显着较高的滴度。接触水平可以部分解释动物饲料加工业工人患肝癌风险增加的原因。
The exposure to aflatoxin B1 (AFB) in animal-feed processing plants was assessed using binding of AFB to serum albumin. The albumin fraction was digested with pronase, and the digest was purified on a C18 Sepak column and an aflatest affinity column before quantification by ELISA. The level of detectability was 5 pg/mg albumin. The workers served as their own controls, as blood samples were taken upon return from vacation and after 4 weeks of work. A total of 7 of 45 samples were positive for AFB, with an estimated average daily intake of 64 ng AFB/kg body weight. The exposed workers had been disembarking cargos contaminated with AFB or working at places where the dust contained detectable amounts of AFB. The sera from the exposed workers had a significantly higher titer against an aflatoxin B1-epitope than a nonexposed Danish control group. The level of exposure could partly explain the increased risk of liver cancer in workers in the animal-feed processing industry.