Kartogenin-Incorporated Thermogel Supports Stem Cells for Significant Cartilage Regeneration
Kartogenin-Incorporated Thermogel Supports Stem Cells for Significant Cartilage Regeneration
复制标题
加入 Kartogenin 的热凝胶支持干细胞显着软骨再生
DOI：
10.1021/acsami.5b12212
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发表时间：
2016-03-02
影响因子：
9.5
通讯作者：
Chen, Xuesi
中科院分区：
文献类型：
--
作者：
Li, Xuezhou;Ding, Jianxun;Chen, Xuesi
近年来，软骨组织工程在软骨缺损修复中的应用受到越来越多的关注。本研究将一种新型的软骨诱导性非蛋白小分子KGN引入到聚(L-丙交酯-聚乙交酯)-聚乙二醇聚(L-丙交酯-乙交酯)热凝胶中，构建了合适的骨髓间充质干细胞的微环境，以实现有效的软骨再生。KGN和BMSCs复合热凝胶组修复的关节表面更完整、更光滑，特征性糖胺多聚糖(GAG)和II型胶原(Col II)含量更丰富，正常软骨变性更少。综上所述，负载KGN的PLGA-PEG-PLGA热凝胶可作为BMSCs体内再生受损软骨的替代载体。
Recently, cartilage tissue engineering (CTE) attracts increasing attention in cartilage defect repair. In this work, kartogenin (KGN), an emerging chondroinductive nonprotein small molecule, was incorporated into a thermogel of poly(L-lactide-co-glycolide)-poly(ethylene glycol)-poly(L-lactide-co-glycolide) (PLGA-PEG-PLGA) to fabricate an appropriate rnicroenvironment of bone marrow mesenchymal stem cells (BMSCs) for effective cartilage regeneration. More integrative and smoother repaired articular surface, more abundant characteristic glycosaminoglycans (GAGS) and collagen II (COL II), and less degeneration of normal cartilage were obtained in the KGN and BMSCs coloaded thermogel group in vivo. In conclusion, the KGN-loaded PLGA-PEG-PLGA thermogel can be utilized as an alternative support for BMSCs to regenerate damaged cartilage in vivo.