Resveratrol Decreases TXNIP mRNA and Protein Nuclear Expressions With an Arterial Function Improvement in Old Mice
Resveratrol Decreases TXNIP mRNA and Protein Nuclear Expressions With an Arterial Function Improvement in Old Mice
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DOI：
10.1093/gerona/glv071
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发表时间：
2016-06-01
影响因子：
5.1
通讯作者：
Nivet-Antoine, Valerie
中科院分区：
文献类型：
--
作者：
Bedarida, Tatiana;Baron, Stephanie;Nivet-Antoine, Valerie
衰老导致葡萄糖耐受不良和心血管疾病的高患病率，氧化应激起着潜在的作用。白藜芦醇已显示出对葡萄糖耐量的有希望的作用，并倾向于改善老年患者的内皮功能。硫氧还蛋白相互作用蛋白（TXNIP）最近被认为是连接葡萄糖代谢和氧化应激的潜在纽带。在这里，我们研究了白藜芦醇诱导的老年小鼠动脉衰老表型的改善和主动脉TXNIP的表达。使用有或没有3个月白藜芦醇治疗的老年小鼠的体内模型，我们通过研究炎症和氧化应激因子，从心血管多普勒分析到分子水平，研究了白藜芦醇对年龄相关性损伤的影响。我们发现了白藜芦醇的双重作用，降低与年龄相关的葡萄糖耐受不良和氧化应激失衡，导致减少基质重塑，从而在内膜中层厚度和动脉扩张性方面阻止动脉老化表型。这些结果提供了第一个证据，主动脉TXNIP mRNA和蛋白核表达增加动脉老化和白藜芦醇治疗下降。总之，我们证明白藜芦醇有助于恢复衰老小鼠的几个受损过程，降低主动脉TXNIP mRNA和蛋白核表达。
Aging leads to a high prevalence of glucose intolerance and cardiovascular diseases, with oxidative stress playing a potential role. Resveratrol has shown promising effects on glucose tolerance and tends to improve endothelial function in elderly patients. Thioredoxin-interacting protein (TXNIP) was recently proposed as a potential link connecting glucose metabolism to oxidative stress. Here, we investigated the resveratrol-induced improvement of arterial aging phenotype in old mice and the expression of aortic TXNIP. Using an in vivo model of old mice with or without 3-month resveratrol treatment, we investigated the effects of resveratrol on age-related impairments from a cardiovascular Doppler analysis, to a molecular level, by studying inflammation and oxidative stress factors. We found a dual effect of resveratrol, with a decrease of age-related glucose intolerance and oxidative stress imbalance leading to reduced matrix remodeling that forestalls arterial aging phenotype in terms of intima-media thickness and arterial distensibility. These results provide the first evidence that aortic TXNIP mRNA and protein nuclear expressions are increased in the arterial aging and decreased by resveratrol treatment. In conclusion, we demonstrated that resveratrol helped to restore several aging impaired processes in old mice, with a decrease of aortic TXNIP mRNA and protein nuclear expressions.