Na+/H+ exchange in primary, secondary and n-butyrate-treated cultures of ruminal epithelial cells:: Short communication
Na+/H+ exchange in primary, secondary and n-butyrate-treated cultures of ruminal epithelial cells:: Short communication
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DOI：
10.1556/avet.50.2002.2.10
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发表时间：
2002-01-01
影响因子：
0.9
通讯作者：
Gäbel, G
中科院分区：
文献类型：
--
作者：
Gálfi, P;Neogrády, S;Gäbel, G
研究了培养的瘤胃上皮细胞对阿米洛利敏感的Na+摄取速率，以阐明培养条件对Na+/H+交换（NHE）的影响。将细胞培养物暴露于或不暴露于正丁酸钠7天或传代培养。在培养的第14天，没有丁酸盐暴露的原代细胞培养物显示出非分层和分层生长。正丁酸钠处理的14日龄培养物和3日龄传代培养物主要含有非分层细胞，即非角化细胞。正丁酸盐处理和传代培养增加总的和阿米洛利敏感的Na+吸收。我们的研究结果表明，通过NHE的Na+吸收是由非分层（非角化）细胞的数量和比例。
Rate of amiloride-sensitive Na+ uptake into cultured rumen epithelial cells was studied in order to clarify the influence of culture conditions on Na+/H+ exchange (NHE). Cell cultures were exposed to Na-n-butyrate or not for seven days or subcultured. On the 14th day of culturing, primary cell cultures without butyrate exposure showed both non-stratified and stratified growth. Na-n-butyrate treated 14-day-old cultures and 3-day-old subcultures contained mostly non-stratified, i.e. non-keratinised cells. Both n-butyrate treatment and subculturing increased total and amiloride-sensitive Na+ uptake. Our results indicate that Na+ uptake via NHE is determined by the amount and the ratio of non-stratified (non-keratinised) cells.