Chemical genetics and drug discovery in Xenopus.
Chemical genetics and drug discovery in Xenopus.
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DOI：
10.1007/978-1-61779-992-1_9
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发表时间：
2012
影响因子：
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通讯作者：
Matthew L. Tomlinson;Adam E. Hendry;G. Wheeler
中科院分区：
文献类型：
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作者：
Matthew L. Tomlinson;Adam E. Hendry;G. Wheeler
化学遗传学使用小分子来调节蛋白质功能，并有可能扰乱复杂细胞环境中的任何生化事件。应用化学遗传学来剖析生物过程，由于其技术简单、试剂便宜和启动成本低，已成为诱变筛选的一种有吸引力的替代方案。非洲爪蟾胚胎特别适合整个生物体化学遗传筛选。在这里，我们描述了我们开发的用于筛选爪蟾胚胎小型化合物库的基本方案。我们通过观察整个蝌蚪的表型变化或使用自动化整体原位杂交基因表达模式的变化来对胚胎进行评分。
Chemical genetics uses small molecules to modulate protein function and has the potential to perturb any biochemical event in a complex cellular context. The application of chemical genetics to dissect biological processes has become an attractive alternative to mutagenesis screens due to its technical simplicity, inexpensive reagents, and low-startup costs.Xenopusembryos are particularly amenable to whole organism chemical genetic screens. Here we describe the basic protocols we have developed to screen small compound libraries onXenopus laevisembryos. We score embryos either by observing phenotypic changes in the whole tadpole or by changes in gene expression pattern using automated wholemount in situ hybridization.