Reticulon 3 is involved in membrane trafficking between the endoplasmic reticulum and Golgi
Reticulon 3 is involved in membrane trafficking between the endoplasmic reticulum and Golgi
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DOI：
10.1016/j.bbrc.2005.07.012
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发表时间：
2005-09-09
影响因子：
3.1
通讯作者：
Tagaya, M
中科院分区：
文献类型：
--
作者：
Wakana, Y;Koyama, S;Tagaya, M
网状蛋白 (RTN) 构成具有网状分布的内质网 (ER) 相关蛋白家族。尽管它们的神经元亚型在轴突再生中具有重要意义，但其广泛表达的亚型的功能在很大程度上是未知的。在这项研究中，我们研究了普遍表达的 RTN3 在膜运输中的作用。异位表达的 RTN3 表现出异质模式；丝状、网状、粒状分布。 ER形态发生相应变化。在 RTN3 显示丝状/网状分布的细胞中，内质网和高尔基体之间的蛋白质转运被阻断，高尔基体蛋白质被分散。相比之下，ERGIC-53（ER-高尔基体中间区室的标记物）在核周区域积聚，甚至在用诱导高尔基体蛋白重新分布至内质网的试剂处理细胞后仍保留在那里，表明ERGIC-53的高尔基体至内质网转运受到抑制。这些结果表明 RTN3 在早期分泌途径的膜运输中发挥作用。 (c) 2005 Elsevier Inc. 保留所有权利。
Reticulons (RTNs) constitute a family of endoplasmic reticulum (ER)-associated proteins with a reticular distribution. Despite the implication of their neuronal isoforms in axonal regeneration, the function of their widely expressed isoforms is largely unknown. In this study, we examined the role of the ubiquitously expressed RTN3 in membrane trafficking. Ectopically expressed RTN3 exhibited heterogeneous patterns; filamentous, reticular, and granular distributions. The ER morphology changed accordingly. In cells where RTN3 displayed a filamentous/reticular distribution, protein transport between the ER and Golgi was blocked, and Golgi proteins were dispersed. In contrast, ERGIC-53, a marker for the ER-Golgi intermediate compartment, accumulated at the perinuclear region, and remained there even after cells were treated with agents that induce redistribution of Golgi proteins to the ER, indicating an inhibition of Golgi-to-ER transport of ERGIC-53. These results suggest that RTN3 plays a role in membrane trafficking in the early secretory pathway. (c) 2005 Elsevier Inc. All rights reserved.