Immunoreactivity to cell surface syndecans in cytoplasm and nucleus: Tubulin-dependent rearrangements
Immunoreactivity to cell surface syndecans in cytoplasm and nucleus: Tubulin-dependent rearrangements
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DOI：
10.1006/excr.2002.5477
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发表时间：
2002-04-01
影响因子：
3.7
通讯作者：
Hjerpe, A
中科院分区：
文献类型：
--
作者：
Brockstedt, U;Dobra, K;Hjerpe, A
多配体聚糖是跨膜蛋白聚糖，通过与生长因子相互作用参与细胞生长和分化的调节。虽然多配体蛋白聚糖在调节细胞形态中起着重要作用，但对其亚细胞分布和体内与细胞骨架的关系知之甚少。为了解决这个问题，我们研究了syndecans-1，-2和-4的亚细胞分布和动态重排，使用共聚焦激光显微镜。此外，我们监测了有丝分裂和间期细胞中多配体蛋白聚糖与微管蛋白的空间关系。最初，对多配体蛋白聚糖的反应性仅限于细胞质，随后出现细胞膜的染色。Syndecan-1似乎也以时间依赖性的方式易位到细胞核。有丝分裂纺锤体显示出乎意料的多syndecans比发现在间期细胞。长春碱处理后，syndecan-1和微管蛋白均恢复为次晶闭塞体，syndecan-1的核反应性消失，表明微管蛋白介导的这种蛋白多糖的核转运。有丝分裂后的细胞出现质膜染色。核转位主要影响syndecan-1，而syndecan-2和-4留在细胞质和细胞膜。这是第一次报告的调节核转位和syndecan-1的存在下，在有丝分裂纺锤体，在那里它可以稳定有丝分裂机器。多配体蛋白聚糖-1/微管蛋白复合物还可以作为将蛋白质生长因子转运到细胞核的载体。(C)2002 Elsevier Science（美国）。
Syndecans are transmembrane proteoglycans implicated in the regulation of cell growth and differentiation, by interacting with growth factors. Although syndecans play a major role in regulating cell morphology, little is known about their subcellular distribution and in vivo association with the cytoskeleton. To address this question, we investigated the subcellular distribution and dynamic rearrangement of syndecans-1, -2, and -4, using confocal laser microscopy. Furthermore, we monitored the spatial relation of syndecans to tubulin in both mitotic and interphase cells. Initially, the reactivity to syndecans was confined to the cytoplasm, staining of the cell membranes appearing later. Syndecan-1 also seems to translocate to the nucleus in a time-dependent manner. The mitotic spindle shows unexpectedly more syndecans than that found in interphase cells. After vinblastine treatment, both syndecan-1 and tubulin were recovered as paracrystalline occlusion bodies, and the nuclear reactivity to syndecan-1 disappeared, suggesting tubulin-mediated nuclear transport of this proteoglycan. Plasma membrane staining reappeared in the postmitotic cells. Nuclear translocation predominantly affected syndecan-1, whereas syndecan-2 and -4 remained in cytoplasm and cell membrane. This is the first report on regulated nuclear translocation and the presence of syndecan-1 in the mitotic spindle, where it may stabilize the mitotic machinery. The syndecan-1/ tubulin complex may also act as a vehicle for the transport of protein growth factors to the cell nucleus. (C) 2002 Elsevier Science (USA).