Identification and genetic characterization by high-throughput SNP analysis of intervarietal substitution lines of rapeseed (Brassica napus L.) with enhanced embryogenic potential
Identification and genetic characterization by high-throughput SNP analysis of intervarietal substitution lines of rapeseed (Brassica napus L.) with enhanced embryogenic potential
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DOI：
10.1007/s00122-015-2455-7
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发表时间：
2015-04-01
影响因子：
5.4
通讯作者：
Hirsch, Ann-Catrin
中科院分区：
文献类型：
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作者：
Ecke, Wolfgang;Kampouridis, Anthimos;Hirsch, Ann-Catrin
鉴定了7个品种间代换系，其胚性潜能是轮回亲本的40.4倍，为进一步深入研究这一性状提供了理想材料。标记分离在单倍体小孢子分离群体中进行了分析，从‘Express 617’和‘RS 239’之间的杂交获得的BC 1群体和衍生的胚胎。在构建图谱后，从与“Express 617”作为轮回亲本的同一杂交中选择了15个品种间代换系，其中供体区段覆盖了在小孢子衍生胚群体中显示出偏斜分离而在BC 1群体中未显示出偏斜分离的5个基因组区域。通过比较15个代换系和“Express 617”的小孢子胚胎发生潜力，鉴定出7个代换系的胚胎发生潜力显着增强，范围为“Express 617”的4.1至40.4倍。为了改善所选品系的遗传表征，使用用于油菜籽的Illumina Infinium 60 K芯片对它们进行高通量SNP分析。在7,960个SNP标记的基础上，每株系发现1 ~ 8个供体片段，占SNP图谱2126.1cM的0.64- 6.79%，证明在代换系群体的发育过程中发生了同源异源交换，增加了群体内的遗传多样性。通过比较具有显著增强的胚胎发生潜力的品系和不显著的品系之间的供体片段，鉴定出12个基因组区域，其可能包含控制油菜胚胎发生潜力的遗传因子。这些区域的大小范围从0（仅由一个标记表示）到26.8cM，并且一起仅覆盖SNP图谱的5.42%。
Seven intervarietal substitution lines were identified with embryogenic potentials up to 40.4 times that of the recurrent parent, providing an ideal material for further in depth studies of this trait.To identify genomic regions that carry genetic factors controlling embryogenic potential of isolated microspores of rapeseed, marker segregations were analysed in a segregating population of haploid microspore-derived embryos and a BC1 population from a cross between 'Express 617' and 'RS239'. After map construction 15 intervarietal substitution lines from the same cross with 'Express 617' as recurrent parent were selected with donor segments covering five genomic regions that had shown skewed segregations in the population of microspore-derived embryos but not in the BC1 population. By comparing the embryogenic potential of microspores of the 15 substitution lines and 'Express 617', seven lines were identified with significantly enhanced embryogenic potential ranging from 4.1 to 40.4 times that of 'Express 617'. To improve the genetic characterization of the selected lines, they were subjected to a high-throughput SNP analysis using the Illumina Infinium 60K chip for rapeseed. Based on 7,960 mapped SNP markers, one to eight donor segments per line, which cover 0.64-6.79 % of the 2,126.1 cM of the SNP map, were found. The SNP analysis also gave evidence that homoeologous exchanges had occurred during the development of the substitution line population, increasing the genetic diversity within this population. By comparing donor segments between lines with significantly enhanced embryogenic potential and non-significant lines, 12 genomic regions were identified that may contain genetic factors controlling embryogenic potential in rapeseed. These regions range in size from 0 (represented by just one marker) to 26.8 cM and cover together just 5.42 % of the SNP map.