Drosophila APC2 and Armadillo participate in tethering mitotic spindles to cortical actin
Drosophila APC2 and Armadillo participate in tethering mitotic spindles to cortical actin
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DOI：
10.1038/ncb1001-933
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发表时间：
2001-10-01
影响因子：
21.3
通讯作者：
Peifer, M
中科院分区：
文献类型：
--
作者：
McCartney, BM;McEwen, DG;Peifer, M
有丝分裂纺锤体的正确定位确保了染色体平等地分配给子细胞。这通常涉及纺锤体和星形微管与皮质肌动蛋白之间的相互作用(1)。在酵母和秀丽线虫中，一些连接纺锤体和皮质的蛋白质机制已经被发现，但在大多数动物细胞中，这一过程仍然是神秘的。在这里，我们报道了肿瘤抑制同系物APC2和它的结合伙伴Armadillo在果蝇早期胚胎合体有丝分裂的纺锤体锚定中都发挥了作用。螳螂、α-连环素和APC2都定位于皮质纺锤体附着的部位。APC2-Armadillo复合体多定位于间期微管。Zust-White 3激酶可以磷酸化Armadillo和APC，也是纺锤体定位的关键，并调节APC2-Armadillo复合体的定位。综上所述，这些数据表明，APC2、Armadillo和α-catenin在纺锤体和皮质肌动蛋白之间提供了重要的联系，并且这种联系受Zust-White 3激酶的调节。
Proper positioning of mitotic spindles ensures equal allocation of chromosomes to daughter cells. This often involves interactions between spindle and astral microtubules and cortical actin(1). In yeast and Caenorhabditis elegans, some of the protein machinery that connects spindles and cortex has been identified but, in most animal cells, this process remains mysterious. Here, we report that the tumour suppresser homologue APC2 and its binding partner Armadillo both play roles in spindle anchoring during the syncytial mitoses of early Drosophila embryos. Armadillo, alpha -catenin and APC2 all localize to sites of cortical spindle attachment. APC2-Armadillo complexes often localize with interphase microtubules. Zeste-white 3 kinase, which can phosphorylate Armadillo and APC, is also crucial for spindle positioning and regulates the localization of APC2-Armadillo complexes. Together, these data suggest that APC2, Armadillo and alpha -catenin provide an important link between spindles and cortical actin, and that this link is regulated by Zeste-white 3 kinase.