Tongxinluo Enhances Neurogenesis and Angiogenesis in Peri-Infarct Area and Subventricular Zone and Promotes Functional Recovery after Focal Cerebral Ischemic Infarction in Hypertensive Rats.
Tongxinluo Enhances Neurogenesis and Angiogenesis in Peri-Infarct Area and Subventricular Zone and Promotes Functional Recovery after Focal Cerebral Ischemic Infarction in Hypertensive Rats.
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通心络增强高血压大鼠梗死周围和室下区神经发生和血管生成促进局灶性脑缺血性梗死后功能恢复
DOI：
10.1155/2016/8549590
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发表时间：
2016
期刊：
影响因子：
--
通讯作者：
Zeng J
中科院分区：
文献类型：
--
作者：
Chen L;Wang X;Zhang J;Dang C;Liu G;Liang Z;Huang G;Zhao W;Zeng J
背景。通心络是一种具有促进脑缺血后神经元功能恢复潜力的中药复方。目标。本研究旨在揭示通心络是否能促进高血压大鼠脑缺血后梗死区及SVZ的神经功能恢复及神经新生和血管新生。方法。采用大脑中远端动脉闭塞（MCAO）法制备高血压大鼠缺血模型。通心络分别于术后24 h给药，持续3、7、14 d。通过行为学试验评价通心络的保护作用。采用免疫化学染色法观察通心络对MCAO模型大鼠神经发生和血管形成的影响。结果。损伤后给药通心络可改善MCAO模型大鼠的神经元功能缺损。免疫化学染色结果显示，通心络给药后，BrdU+/DCX+、BrdU+/nestin+、BrdU+血管内皮细胞在SVZ中增殖增强。通心络给药后，观察梗死周围神经细胞的NeuN染色和层粘连蛋白染色。结论。通心络缺血后给药能改善模型大鼠的神经功能缺损。可能的机制与梗死周围区和SVZ的神经发生和血管生成有关。
Background. Tongxinluo is a traditional Chinese medicine compound with the potential to promote the neuronal functional recovery in cerebral ischemic infarction. Objective. This study aimed to disclose whether tongxinluo promotes neurological functional recovery and neurogenesis and angiogenesis in the infarcted area and SVZ after cerebral ischemic infarction in hypertensive rats. Methods. The ischemic model was prepared by distal middle cerebral artery occlusion (MCAO) in hypertensive rats. Tongxinluo was administrated 24 h after MCAO and lasted for 3, 7, or 14 days. Behavioral tests were performed to evaluate the protection of tongxinluo. Immunochemical staining was applied on brain tissue to evaluate the effects of tongxinluo on neurogenesis and vascularization in the MCAO model rats. Results. Postinjury administration of tongxinluo ameliorated the neuronal function deficit in the MCAO model rats. As evidenced by the immunochemical staining, BrdU+/DCX+, BrdU+/nestin+, and BrdU+ vascular endothelial cells were promoted to proliferate in SVZ after tongxinluo administration. The matured neurons stained by NeuN and vascularization by laminin staining were observed after tongxinluo administration in the peri-infarct area. Conclusion. Tongxinluo postischemia administration could ameliorate the neurological function deficit in the model rats. Possible mechanisms are related to neurogenesis and angiogenesis in the peri-infarct area and SVZ.