Diversity, Bacterial Symbionts and Antibacterial Potential of Gut-Associated Fungi Isolated from the Pantala flavescens Larvae in China.
Diversity, Bacterial Symbionts and Antibacterial Potential of Gut-Associated Fungi Isolated from the Pantala flavescens Larvae in China.
复制标题
DOI：
10.1371/journal.pone.0134542
复制
发表时间：
2015
期刊：
影响因子：
3.7
通讯作者：
Zhang YL
中科院分区：
文献类型：
--
作者：
Shao MW;Lu YH;Miao S;Zhang Y;Chen TT;Zhang YL
采用培养依赖法和基于内部转录间隔序列分析的分子鉴定方法，研究了黄颡鱼（Pantala flavescens）幼虫肠道相关真菌的多样性。从黄颡鱼幼虫中分离到48株真菌。根据系统发育分析，分离真菌可归为5纲12属。从真菌菌丝的全基因组DNA提取中获得了14个细菌16S rDNA序列。大部分序列与变形菌门（13/14）相关，包括1个杆菌科（1/14）。乳酸菌（Leclercia sp.）、oncorhynchi海洋杆菌（Oceanobacillus oncorhynchi）和外施甲基杆菌（Methylobacterium extorquens）是首次报道的真菌内共生细菌。高效液相色谱（HPLC）分析表明，细菌共生体产生特定代谢物，并对真菌代谢物有抑制作用。研究了真菌培养提取物对病原菌金黄色葡萄球菌（ATCC 6538）、枯草芽孢杆菌（ATCC 6633）和大肠杆菌（ATCC 8739）的抑菌活性，其中20种（42%）提取物对至少一种细菌具有抑菌活性。本研究首次报道了黄斑霉幼虫肠道内共生真菌的多样性和抗菌活性，结果表明这些真菌具有高度的多样性，可以作为生物活性化合物的潜在来源。
The diversity of fungi associated with the gut of Pantala flavescens larvae was investigated using a culture-dependent method and molecular identification based on an analysis of the internally transcribed spacer sequence. In total, 48 fungal isolates were obtained from P. flavescens larvae. Based on phylogenetic analyses, the fungal isolates were grouped in 5 classes and 12 different genera. Fourteen bacterial 16S rDNA sequences derived from total genomic DNA extractions of fungal mycelia were obtained. The majority of the sequences were associated with Proteobacteria (13/14), and one Bacillaceae (1/14) was included. Leclercia sp., Oceanobacillus oncorhynchi and Methylobacterium extorquens, were reported for the first time as bacterial endosymbionts in fungi. High-performance liquid chromatography (HPLC) analysis indicated that bacterial symbionts produced specific metabolites and also exerted an inhibitory effect on fungal metabolites. The biological activity of the fungal culture extracts against the pathogenic bacteria Staphylococcus aureus (ATCC 6538), Bacillus subtilis (ATCC 6633) and Escherichia coli (ATCC 8739) was investigated, and 20 extracts (42%) exhibited antibacterial activity against at least one of the tested bacterial strains. This study is the first report on the diversity and antibacterial activity of symbiotic fungi residing in the gut of P. flavescens larvae, and the results show that these fungi are highly diverse and could be exploited as a potential source of bioactive compounds.