pT3.2I, the smallest plasmid of Thiobacillus T3.2.
pT3.2I, the smallest plasmid of Thiobacillus T3.2.
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pT3.2I，硫杆菌T3.2的最小质粒。
DOI：
10.1006/plas.2000.1461
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发表时间：
2000
期刊：
影响因子：
2.6
通讯作者：
J. Perera
中科院分区：
文献类型：
--
作者：
T. Aparicio;P. Lorenzo;J. Perera
嗜酸细菌硫杆菌T3.2的最小质粒pT3.2I的全核苷酸序列已被测定。 pT3.2I 长度为 15,390 bp，GC 含量为 53.7%。其中可以定义不同的区域：一个2569 bp的推定插入序列，与某些农杆菌Ti质粒的其他插入序列相似；另一个序列更长，存在于两个几乎相同的拷贝中，仅删除了 1 bp（6406 和 6405 bp）。在这个重复区域中发现了几个开放阅读框和一些较小的序列：ORFA和ORFG，分别编码推定的多元醇脱氢酶和推定的RepA复制蛋白，可以编码反义RNA的83 bp序列，以及与ColE2和ColE3相关质粒的ori序列高度同源的36 bp区域。另一个推定基因 ORFH 仅存在于该区域的较长拷贝中（在短拷贝中被删除），并且可能编码 90 个氨基酸的多肽，该多肽可充当第二个复制蛋白 RepB。基于序列比较，pT3。 2I可以与pColE2-CA42 incB不相容组中的质粒相关。
The whole nucleotide sequence of pT3.2I, the smallest plasmid of the acidophilic bacterium Thiobacillus T3.2, has been determined. pT3.2I is 15,390 bp long with a 53.7% GC content. Different regions can be defined in it: one 2569-bp putative insertion sequence similar to other insertion sequences of some Agrobacterium Ti plasmids; and a longer sequence, which occurs in two almost identical copies, differing only in a 1-bp deletion (6406 and 6405 bp). Several open reading frames and some smaller sequences were found in this duplicated region: ORFA and ORFG, encoding a putative polyol dehydrogenase and a putative RepA replication protein, respectively, an 83-bp sequence which could code for an antisense RNA, and a 36-bp region highly homologous to ori sequences of ColE2- and ColE3-related plasmids. Another putative gene, ORFH, is only present in the longer copy of this region (it is deleted in the short copy) and might encode a 90-amino-acid polypeptide which could act as a second replication protein, RepB. Based on sequence comparisons, pT3. 2I can be related to plasmids in the pColE2-CA42 incB incompatibility group.