Potential cytoplasmic inheritance in Wisteria sinensis and Robinia pseudoacacia (Leguminosae).
Potential cytoplasmic inheritance in Wisteria sinensis and Robinia pseudoacacia (Leguminosae).
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DOI：
10.1093/pcp/pci110
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发表时间：
2005-07
影响因子：
4.9
通讯作者：
Yufei Hu;Quan Zhang;Sodmergen
中科院分区：
文献类型：
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作者：
Yufei Hu;Quan Zhang;Sodmergen
我们研究了紫藤和刺槐的花粉细胞，以确定这些物种的细胞质遗传的可能模式。落射荧光显微镜显示不同的成熟生殖细胞。W.中华绒螯蟹生殖细胞内出现大量的荧光信号，而中华绒螯蟹生殖细胞内未见荧光信号。刺槐。进一步的检测表明，点状荧光信号对应于质体而不是线粒体DNA。这些结果表明，在W。sinensis。电子显微镜证实了W. sinensis和R.由于在第一次花粉有丝分裂期间质体的不均匀分布，导致了刺槐细胞的分裂。线粒体存在于两个物种的成熟生殖细胞中，并且是完整的。荧光线粒体DNA的缺乏表明细胞中线粒体DNA的水平非常低。免疫电镜观察表明，这些细胞中的线粒体DNA的标记减少了近90%的花粉发育过程中。这种急剧减少表明父亲线粒体DNA的主动降解，这可能极大地促进了线粒体的母系遗传。简而言之，我们发现W。sinensis表现出很强的质体父系传递潜力，W. sinensis和R.刺槐似乎共享相同的机制为母系线粒体遗传。
We examined pollen cells of Wisteria sinensis and Robinia pseudoacacia (Leguminosae) to determine a possible mode for cytoplasmic inheritance in these species. Epifluorescence microscopy revealed distinct mature generative cells. Mature generative cells of W. sinensis were associated with large numbers of punctuated fluorescent signals corresponding to cytoplasmic DNA aggregates, but no fluorescent signals were observed in the generative cells of R. pseudoacacia. Closer examination showed that the punctate fluorescent signals corresponded to plastid but not mitochondrial DNA. These results suggest a strong potential for paternal transmission of the plastid genome in W. sinensis. Electron microscopy confirmed the presence of plastids in the generative cells of W. sinensis and the absence of plastids in R. pseudoacacia cells due to an unequal distribution of plastids during the first pollen mitosis. Mitochondria were present and intact in the mature generative cells of both species. The lack of fluoresced mitochondrial DNA suggests a very low level of mitochondrial DNA in the cells. Immunoelectron microscopy demonstrated that the labeling of mitochondrial DNA in these cells was reduced by nearly 90% during pollen development. Such a dramatic reduction suggests an active degradation of paternal mitochondrial DNA, which may contribute greatly to the maternal inheritance of mitochondria. In short, we found that W. sinensis exhibits a strong potential for paternal transmission of plastids and that both W. sinensis and R. pseudoacacia appear to share the same mechanism for maternal mitochondrial inheritance.