CLONING OF A HUMAN-LIVER MICROSOMAL UDP-GLUCURONOSYLTRANSFERASE CDNA
CLONING OF A HUMAN-LIVER MICROSOMAL UDP-GLUCURONOSYLTRANSFERASE CDNA
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DOI：
10.1042/bj2420581
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发表时间：
1987-03-01
影响因子：
4.1
通讯作者：
BURCHELL, B
中科院分区：
文献类型：
--
作者：
JACKSON, MR;MCCARTHY, LR;BURCHELL, B
A cDNA clone (HLUG 25) encoding the complete sequence of a human liver UDP-glucuronosyltransferase was isolated from a .lambda.gt 11 human liver cDNA library. The library was screened by hybridization to a partial-length human UDP-glucuronosyltransferase cDNA (pHUDPGT1) identified from a human liver pEX cDNA expression library by using anti-UDP-glucuronosyltransferase antibodies. The authenticity of the cDNA clone was confirmed by hybrid-select translation and extensive sequence homology to rat liver UDP-glucuronosyltransferase cDNAs. The sequence of HLUG 25 cDNA was determined to be 2104 base-pairs long, including a poly(A) tail, and contains a long open reading frame. The possible site of translation initiation of this sequence is discussed with reference to a rat UDP-glucuronosyltransferase cDNA clone (RLUG 38).
