A DEVELOPMENTALLY REGULATED ACTIVITY THAT UNWINDS RNA DUPLEXES
A DEVELOPMENTALLY REGULATED ACTIVITY THAT UNWINDS RNA DUPLEXES
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DOI：
10.1016/0092-8674(87)90239-x
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发表时间：
1987-02-27
期刊：
影响因子：
64.5
通讯作者：
WEINTRAUB, H
中科院分区：
文献类型：
--
作者：
BASS, BL;WEINTRAUB, H
我们曾试图在非洲爪蟾胚胎发育中使用反义RNA技术，发现尽管正义和反义RNA之间形成杂交，但它们是短暂的。我们的研究表明，这是由于存在一种使RNA：RNA杂交体解旋的活性。该活性在爪蟾卵母细胞中以低但可检测的水平存在，在卵母细胞成熟期间增加，并且在整个早期胚胎发生中以高水平存在。活性已在S100提取物中表征。特异性RNA：RNA杂交体的变性被第二个双链RNA的加入所抑制，但不被单链RNA、单链DNA或双链DNA的加入所抑制。
We have attempted to use antisense RNA techniques in the developing Xenopus embryo and found that although hybrids form between sense and antisense RNAs, they are transient. Our studies indicate that this is due to the presence of an activity that unwinds RNA:RNA hybrids. The activity is present at low, but detectable, levels in Xenopus oocytes, increases during oocyte maturation, and exists at high levels throughout early embryogenesis. The activity has been characterized in S100 extracts. The denaturation of a specific RNA:RNA hybrid is inhibited by the addition of a second duplexed RNA but not by the addition of single-stranded RNA, single-stranded DNA, or double-stranded DNA.