Calcium signaling contributes to xylem vessel cell differentiation via post-transcriptional regulation of VND7 downstream events
Calcium signaling contributes to xylem vessel cell differentiation via post-transcriptional regulation of VND7 downstream events
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DOI：
10.5511/plantbiotechnology.21.0519a
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发表时间：
2021-09-01
影响因子：
1.6
通讯作者：
Ohtani, Misato
中科院分区：
文献类型：
--
作者：
Kamon, Eri;Noda, Chihiro;Ohtani, Misato
次生细胞壁（SCW）积累在维管植物的特定细胞类型，特别是木质部导管细胞。以前的研究表明，钙离子（Ca 2+）参与木质部导管细胞分化，但它们是否在SCW沉积功能尚不清楚。在这项研究中，我们研究了在木质部导管细胞分化过程中，使用拟南芥悬浮培养的细胞携带的VND 7诱导系统，其中VND 7活性可以后诱导上调，以诱导转分化成protoxylem型导管细胞的SCW沉积中的作用。我们观察到，细胞外Ca 2+浓度是分化的一个重要决定因素，尽管它对VND 7下游基因的转录没有一致的影响。增加Ca ~（2+）浓度可降低细胞的分化，但细胞可形成螺旋状的螺旋纹。暴露于钙通道抑制剂部分恢复分化，但导致异常分支和网状SCW图案。这些数据表明，Ca 2+信号参与木质部导管细胞分化通过转录后调控的VND 7下游事件，如图案的SCW沉积。
Secondary cell walls (SCWs) accumulate in specific cell types of vascular plants, notably xylem vessel cells. Previous work has shown that calcium ions (Ca2+) participate in xylem vessel cell differentiation, but whether they function in SCW deposition remains unclear. In this study, we examined the role of Ca2+ in SCW deposition during xylem vessel cell differentiation using Arabidopsis thaliana suspension-cultured cells carrying the VND7-inducible system, in which VND7 activity can be post-translationally upregulated to induce transdifferentiation into protoxylem-type vessel cells. We observed that extracellular Ca2+ concentration was a crucial determinant of differentiation, although it did not have consistent effects on the transcription of VND7-downstream genes as a whole. Increasing the Ca2+ concentration reduced differentiation but the cells could generate the spiral patterning of SCWs. Exposure to a calcium-channel inhibitor partly restored differentiation but resulted in abnormal branched and net-like SCW patterning. These data suggest that Ca2+ signaling participates in xylem vessel cell differentiation via post-transcriptional regulation of VND7-downstream events, such as patterning of SCW deposition.