Analysis of Serotonin in Human Feces Using Solid Phase Extraction and Column-Switching LC-MS/MS
Analysis of Serotonin in Human Feces Using Solid Phase Extraction and Column-Switching LC-MS/MS
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DOI：
10.5702/massspectrometry.a0081
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发表时间：
2020-03
影响因子：
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通讯作者：
Y. Hirabayashi;Kiminori Nakamura;Tsuyoshi Sonehara;D. Suzuki;Satoru Hanzawa;Yu Shimizu;T. Aizawa;Koshi Nakamura;A. Tamakoshi;T. Ayabe
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文献类型：
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作者：
Y. Hirabayashi;Kiminori Nakamura;Tsuyoshi Sonehara;D. Suzuki;Satoru Hanzawa;Yu Shimizu;T. Aizawa;Koshi Nakamura;A. Tamakoshi;T. Ayabe
血清素是一种重要的神经递质，主要在肠道中产生，粪便中的血清素水平可以作为肠道环境的指标。然而，人粪便中含有大量污染物，由于食物内容物和肠道环境的不同，这些污染物的变化很大，预计这些污染物会干扰人粪便中5-羟色胺水平的测定。为了去除这些污染物，并确定血清素水平，我们开发了一种新的方法，使用固相萃取（SPE）和柱切换LC-MS/MS。血清素，用稳定的同位素标记，加入到人粪便样品前SPE作为内标，以纠正基质效应的个体差异。两名受试者粪便中SPE的回收率为55.9-81.0%（日内）和56.5-78.1%（日间）。我们分析了来自96名受试者的220份粪便样本，其中包括76名孕妇和产后妇女。孕妇和产后妇女每单位干重粪便的内源性5-羟色胺含量为0.09-14.13 ng/mg，其余受试者为0.30-9.93 ng/mg。
Serotonin, an important neurotransmitter, is produced mainly in intestines, and serotonin levels in feces can be an indicator of the intestinal environment. Human feces, however, contain a large amount of contaminants, which vary widely owing to food contents and the intestinal environment, and these contaminants would be expected to interfere with the determination of serotonin levels in human feces. To remove these contaminants and determine serotonin levels, we developed a new method using solid phase extraction (SPE) and column-switching LC-MS/MS. Serotonin, labeled with a stable isotope, was added to human feces samples prior to SPE as an internal standard to correct for individual differences in matrix effects. The recovery rate for SPE was 55.9–81.0% (intraday) and 56.5–78.1% (interday) for feces from two subjects. We analyzed 220 fecal samples from 96 subjects including 76 pregnant and post-delivery women. The endogenous serotonin content per unit weight of dried feces was 0.09–14.13 ng/mg for pregnant and post-delivery women and 0.30–9.93 ng/mg for the remaining subjects.