PHOTODYNAMIC THERAPY OF THE NORMAL RAT STOMACH - A COMPARATIVE-STUDY BETWEEN DI-SULFONATED ALUMINUM PHTHALOCYANINE AND 5-AMINOLEVULINIC ACID
PHOTODYNAMIC THERAPY OF THE NORMAL RAT STOMACH - A COMPARATIVE-STUDY BETWEEN DI-SULFONATED ALUMINUM PHTHALOCYANINE AND 5-AMINOLEVULINIC ACID
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DOI：
10.1038/bjc.1992.295
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发表时间：
1992-09-01
影响因子：
8.8
通讯作者：
BOWN, SG
中科院分区：
文献类型：
--
作者：
LOH, CS;BEDWELL, J;BOWN, SG
上消化道的异型增生具有侵袭性恶性变化的风险。手术切除受影响的器官是唯一可用的治疗方法。光动力疗法已被证明是有前途的治疗早期和浅表肿瘤，并可能是有用的切除粘膜发育不良。由于疾病的扩散性质，这种治疗必然涉及大面积粘膜的破坏，并且希望将其作用限制在粘膜，以便可以发生安全愈合。本文用荧光光度显微镜研究了正常大鼠胃中二磺酸铝酞菁（AlS_2 Pc）和5-氨基乙酰丙酸（ALA）的光敏化作用。AlS 2 Pc导致胃壁的全壁光敏化，在粘膜下层中的水平最高。粘膜和固有肌层致敏程度相同。在光暴露之后，导致全厚度损伤。ALA是一种天然卟啉前体，外源性给药引起原卟啉IX（PPIX）在细胞中的积累。由此产生的模式的光敏化主要是粘膜和其光动力学效应基本上局限于粘膜。ALA产生胃粘膜的选择性光敏化，用于其光动力学消融，而保留下层组织层。
Dysplasia in the upper gastrointestinal tract carries a risk of invasive malignant change. Surgical excision of the affected organ is the only treatment available. Photodynamic therapy has been shown to be promising in the treatment of early and superficial tumours and may be useful for the ablation of dysplastic mucosa. Because of the diffuse nature of the disease, such treatment would necessarily involve destruction of large areas of mucosa and it is desirable to confine its effect to the mucosa in order that safe healing can take place. By means of photometric fluorescence microscopy, we have studied the pattern of photosensitisation in the normal rat stomach using di-sulphonated aluminium phthalocyanine (AlS2Pc) and 5-aminolaevulinic acid (ALA) as photosensitisers. AlS2Pc resulted in a panmural photosensitisation of the gastric wall with the highest level encountered in the submucosa. The mucosa and muscularis propria were sensitised to equal extent. Following light exposure, a full thickness damage resulted. ALA is a natural porphyrin precursor and exogenous administration gave rise to accumulation of protoporphyrin IX (PPIX) in the cells. The resultant pattern of photosensitisation was predominantly mucosal and its photodynamic effect was essentially confined to the mucosa. ALA produced a selective photosensitisation of the gastric mucosa for its photodynamic ablation with sparing the underlying tissue layers.