Transcriptomic analysis of light-dependent anthocyanin accumulation in bicolored cherry fruits
Transcriptomic analysis of light-dependent anthocyanin accumulation in bicolored cherry fruits
复制标题
双色樱桃果实光依赖性花青素积累的转录组分析
DOI：
10.1016/j.plaphy.2018.08.016
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发表时间：
2018-09-01
影响因子：
6.5
通讯作者：
Li, Tianhong
中科院分区：
文献类型：
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作者：
Guo, Xiao;Wang, Yantao;Li, Tianhong
甜樱桃（Prunus avium L.）果实根据其花色素苷含量分为深红色和双色品种;然而，调节这些色素积累的机制尚不清楚。在这里，我们揭示了花色苷的积累是高度依赖于光的双色'雷尼尔'樱桃，而它只是轻微的光依赖于暗红色的'红灯'果实。为了揭示调控双色'雷尼尔'樱桃中光依赖性花青素积累的转录机制，我们对在光照或黑暗中生长的果实的转录组进行了测序。在双色果实中，编码花色素苷生物合成酶查尔酮合酶、查尔酮异构酶、黄烷酮3-羟化酶和类黄酮3 '-羟化酶的基因被光显著上调。已知大多数差异表达的调控基因参与光或激素信号转导途径，如编码蛋白磷酸酶2Cs、光敏色素相互作用因子3、光敏色素和伸长下胚轴5的基因。在双色果实中发现32个高表达转录因子的表达水平被光显著改变，包括碱性亮氨酸拉链、R2 R3-MYB和WRKY转录因子家族的成员。共表达网络分析进一步揭示了许多光调控基因与参与脱落酸和赤霉酸信号通路的基因共表达，表明这些植物激素在光依赖的花青素合成中起重要作用。总之，我们的数据揭示了光在调节不同颜色樱桃中花青素生物合成中的多种作用。
Sweet cherry (Prunus avium L.) fruits are classified into dark-red and bicolored cultivars based on their anthocyanin contents; however, the mechanisms regulating the accumulation of these pigments are unclear. Here, we reveal that anthocyanin accumulation is highly dependent on light in bicolored 'Rainier' cherries, while it is only slightly light dependent in the dark-red 'Hongdeng' fruits. To reveal the transcriptional mechanisms regulating light-dependent anthocyanin accumulation in bicolored 'Rainier' cherries, we sequenced the transcriptomes of fruits grown in light or in darkness. Genes encoding the anthocyanin biosynthesis enzymes chalcone synthase, chalcone isomerase, flavanone 3-hydroxylase, and flavonoid 3'-hydroxylase were significantly upregulated by light in the bicolored fruits. Most of the differentially expressed regulatory genes were known to be involved in the light or hormone signal transduction pathways, such as those encoding protein phosphatase 2Cs, PHYTOCHROME INTERACTING FACTOR 3, phytochromes, and ELONGATED HYPOCOTYL 5. The expression levels of 32 highly expressed transcription factors were found to be significantly altered by light in the bicolored fruits, including members of the basic leucine zipper, R2R3-MYB, and WRKY transcription factor families. A co-expression network analysis further revealed that many of the light-regulated genes were co-expressed with genes involved in the abscisic acid and gibberellic acid signaling pathways, suggesting that these phytohormones play important roles in light-dependent anthocyanin biosynthesis. Together, our data reveal multiple roles for light in regulating anthocyanin biosynthesis in differently colored cherries.