Beta class amino methyltransferases from bacteria to humans: evolution and structural consequences.
Beta class amino methyltransferases from bacteria to humans: evolution and structural consequences.
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DOI：
10.1093/nar/gkaa446
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发表时间：
2020-10-09
影响因子：
14.9
通讯作者：
Cheng X
中科院分区：
文献类型：
--
作者：
Woodcock CB;Horton JR;Zhang X;Blumenthal RM;Cheng X
s -腺苷-l-蛋氨酸依赖的甲基转移酶催化甲基转移到各种各样的靶分子上，包括DNA和RNA。我们讨论了一类甲基转移酶，它们作用于DNA中的腺嘌呤或胞嘧啶的氨基，在其氨基酸序列中具有特定顺序的保守基序，并被称为β MTases。这一类的成员包括来自大肠杆菌的m.e ecogii和m.e ecop15i，新月形茎杆菌细胞周期调节DNA甲基转移酶（CcrM），来自纤毛虫的MTA1-MTA9复合体，以及来自哺乳动物的MettL3-MettL14复合体。这些甲基转移酶都在DNA中产生n6 -甲基腺嘌呤，其中一些成员在单链DNA和RNA上都有活性。β类甲基转移酶具有独特的多聚体特征，形成同源或异源二聚体，允许酶在底物识别和甲基化方面在两个亚基之间进行分工。我们认为m.c ecogii可能代表了这些酶的祖先形式，因为它的活性独立于核酸类型（RNA或DNA）、它的链结性（单或双）和它的序列（除了目标腺嘌呤）。
S-adenosyl-l-methionine dependent methyltransferases catalyze methyl transfers onto a wide variety of target molecules, including DNA and RNA. We discuss a family of methyltransferases, those that act on the amino groups of adenine or cytosine in DNA, have conserved motifs in a particular order in their amino acid sequence, and are referred to as class beta MTases. Members of this class include M.EcoGII and M.EcoP15I from Escherichia coli, Caulobacter crescentus cell cycle–regulated DNA methyltransferase (CcrM), the MTA1-MTA9 complex from the ciliate Oxytricha, and the mammalian MettL3-MettL14 complex. These methyltransferases all generate N6-methyladenine in DNA, with some members having activity on single-stranded DNA as well as RNA. The beta class of methyltransferases has a unique multimeric feature, forming either homo- or hetero-dimers, allowing the enzyme to use division of labor between two subunits in terms of substrate recognition and methylation. We suggest that M.EcoGII may represent an ancestral form of these enzymes, as its activity is independent of the nucleic acid type (RNA or DNA), its strandedness (single or double), and its sequence (aside from the target adenine).
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影响因子：
3.3
作者：
Barel, Itay;Naughton, Brigitte;Brown, Frank L. H.
通讯作者：
Brown, Frank L. H.
影响因子：
3.3
作者：
Aranda, Juan;Roca, Maite;Tunon, Inaki
通讯作者：
Tunon, Inaki
III型限制性核酸内切酶是异三聚体：包含一个仅结合一个特定DNA的二聚体甲基核酸酶亚基和一个二聚体甲基转移酶。
影响因子：
14.9
作者：
Butterer A;Pernstich C;Smith RM;Sobott F;Szczelkun MD;Tóth J
通讯作者：
Tóth J
影响因子：
5.6
作者：
BESTOR, T;LAUDANO, A;INGRAM, V
通讯作者：
INGRAM, V
影响因子：
8.6
作者：
Clifton, Ben E.;Jackson, Colin J.
通讯作者：
Jackson, Colin J.