jYCaMP: an optimized calcium indicator for two-photon imaging at fiber laser wavelengths
jYCaMP: an optimized calcium indicator for two-photon imaging at fiber laser wavelengths
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DOI：
10.1038/s41592-020-0835-7
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发表时间：
2020-05-25
期刊：
影响因子：
48
通讯作者：
Podgorski, Kaspar
中科院分区：
文献类型：
--
作者：
Mohr, Manuel Alexander;Bushey, Daniel;Podgorski, Kaspar
jYCaMP1是钙指示剂jGCaMP7的黄色变体，可在激发波长超过1,000 nm的情况下实现快速多色双光子成像，用于流行的掺镱光纤和锁模半导体激光器。在1000纳米以上的固定波长的飞秒激光器是强大的，稳定的和廉价的，使它们成为双光子显微镜的有前途的光源。下一代显微镜和价格合理的交钥匙系统都需要针对这些波长进行优化的生物传感器。在这里，我们报道了钙指示剂jGCaMP7的黄色变体jYCaMP1，它在1000 nm以上的激发波长下在小鼠和果蝇中表现优于其亲本，并且能够使用基于红色荧光蛋白的指示剂改善双色钙成像。
jYCaMP1, a yellow variant of the calcium indicator jGCaMP7, enables fast multicolor two-photon imaging at excitation wavelengths above 1,000 nm for use with popular ytterbium-doped fiber and modelocked semiconductor lasers.Femtosecond lasers at fixed wavelengths above 1,000 nm are powerful, stable and inexpensive, making them promising sources for two-photon microscopy. Biosensors optimized for these wavelengths are needed for both next-generation microscopes and affordable turn-key systems. Here we report jYCaMP1, a yellow variant of the calcium indicator jGCaMP7 that outperforms its parent in mice and flies at excitation wavelengths above 1,000 nm and enables improved two-color calcium imaging with red fluorescent protein-based indicators.