miR156a-targeted SBP-Box transcription factor SlSPL13 regulates inflorescence morphogenesis by directly activating SFT in tomato
miR156a-targeted SBP-Box transcription factor SlSPL13 regulates inflorescence morphogenesis by directly activating SFT in tomato
复制标题
miR156a 靶向的 SBP-Box 转录因子 SlSPL13 通过直接激活番茄中的 SFT 来调节花序形态发生。
DOI：
10.1111/pbi.13331
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发表时间：
2020-01-25
影响因子：
13.8
通讯作者：
Zhang, Junhong
中科院分区：
文献类型：
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作者：
Cui, Long;Zheng, Fangyan;Zhang, Junhong
高等植物的花序和侧枝是由侧生分生组织产生的。花序的结构对番茄（Solanum lycopersicum）果实产量有直接影响。我们以前证明，miR 156 a通过抑制SQUAMOSA启动子结合蛋白样（SPL）转录因子基因家族的表达，在决定番茄花序和侧枝的结构中起着重要作用。然而，与花序形态发生相关的调控途径的信息仍然缺乏。在这项研究中，我们证明SPL 13是参与miR 156 a调控的番茄花序结构决定和侧分支产生的主要SPL。抑制番茄中SPL 13的表达增加了营养枝和侧枝上的花序数量，减少了花和果实的数量，并降低了果实的大小和产量。遗传和生化证据表明，SPL 13控制花序发育的番茄花序相关基因单花TRUSS（SFT）的表达，通过直接结合到其启动子区的积极调节。因此，我们的研究结果为我们理解基于miR 156 a-SlSPL的调节番茄植物结构和产量的机制提供了重大进展。
The inflorescences and lateral branches of higher plants are generated by lateral meristems. The structure of the inflorescence has a direct effect on fruit yield in tomato (Solanum lycopersicum). We previously demonstrated that miR156a plays important roles in determining the structures of the inflorescences and lateral branches in tomato by suppressing the expression of the SQUAMOSA PROMOTER BINDING PROTEIN LIKE (SPL) transcription factor gene family. However, information on regulatory pathways associated with inflorescence morphogenesis is still lacking. In this study, we demonstrate that SPL13 is the major SPL involved in miR156a-regulated tomato inflorescence structure determination and lateral branch production. Suppressing the expression of SPL13 in tomato increases the number of inflorescences on vegetative branches and lateral branches, decreases the number of flowers and fruit, and reduces fruit size and yield. Genetic and biochemical evidence indicate that SPL13 controls inflorescence development by positively regulating the expression of the tomato inflorescence-associated gene SINGLE FLOWER TRUSS (SFT) by directly binding to its promoter region. Thus, our findings provide a major advance to our understanding of the miR156a-SlSPL-based mechanism that regulates plant architecture and yield in tomato.