Effect of Formula Composition on the Development of Infant Gut Microbiota
Effect of Formula Composition on the Development of Infant Gut Microbiota
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DOI：
10.1097/mpg.0b013e3182105850
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发表时间：
2011-06-01
影响因子：
2.9
通讯作者：
Steenhout, Philippe G.
中科院分区：
文献类型：
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作者：
Hascoet, Jean-Michel;Hubert, Claire;Steenhout, Philippe G.
目的：母乳喂养会产生富含双歧杆菌的肠道微生物群，而配方奶喂养的婴儿则具有更加多样化的定植。该生态系统有助于母乳喂养婴儿免疫反应的发展以及降低腹泻和过敏的发生率。这项随机双盲对照试验旨在评估以乳清蛋白为主、低磷酸盐和蛋白质的研究配方奶粉的双歧效应，使其成分更接近母乳。 患者和方法：190 名健康婴儿仅接受含有或不含长双歧杆菌 (BL999) 的研究配方奶粉，或长达 4 个月的对照配方奶粉。母乳喂养的婴儿作为参考人群。对 2 个月大时收集的粪便样本进行细菌计数分析（对数菌落形成单位 [CFU]/g）。结果：单独接受研究配方奶粉的婴儿（10.0[0.8]，P < 0.0001，中位数[四分位距]）或接受 BL999（9.8[1.4]，P < 0.01）的双歧杆菌计数显着高于对照婴儿(9.2[3.5])，与母乳喂养婴儿相似(10.1[0.4]，P > 0.05)。 2 个研究组之间的差异为 0.16 log CFU/g（90% 置信区间 [CI] [0-0.4]），在预定义的等效裕度内。微生物群分布（以细菌总数的百分比表示）显示，研究配方奶粉和母乳喂养的婴儿中大约有 50% 双歧杆菌、8% 肠杆菌和 < 10% 梭菌，而对照组分别为 22%、13% 和 19%。各组之间的生长测量、消化耐受性和不良事件没有显着差异。结论：这项研究表明，更接近母乳的婴儿配方奶粉比对照配方奶粉具有更强的双歧杆菌活性，并且产生与母乳喂养婴儿相似的微生物群特征。
Objectives: Breast-feeding induces a gut microbiota rich in bifidobacteria, whereas formula-fed babies have a more diverse colonization. This ecosystem contributes to the development of the immune response and the lower incidence of diarrhea and allergy in breast-fed infants. This randomized double-blind controlled trial aimed to evaluate the bifidogenic effect of a mainly whey protein study formula low in phosphate and protein, allowing a composition closer to that of human milk.Patients and Methods: One hundred ninety healthy infants exclusively received study formula with or without Bifidobacterium longum (BL999), or a control formula for up to 4 months. Breast-fed infants served as a reference population. Stool samples collected at 2 months of age were analyzed for bacterial counts (log colony-forming unit [CFU]/g).Results: Bifidobacteria counts were significantly higher in infants receiving the study formula alone (10.0[0.8], P < 0.0001, median [interquartile range]) or with BL999 (9.8[1.4], P < 0.01) than control (9.2[3.5]), and were similar to breast-fed infants (10.1[0.4], P > 0.05). The difference between the 2 study groups was 0.16 log CFU/g (90% confidence interval [CI] [0-0.4]), within the predefined equivalence margin. Microbiota profile, as a percentage of total bacteria counts, showed about 50% Bifidobacteria, 8% Enterobacteria, and < 10% Clostridia in study formulae and breast-fed infants versus 22%, 13%, and 19% in controls, respectively. There were no significant differences in growth measurements, digestive tolerance, and adverse events between groups.Conclusions: This study showed that infant formula closer resembling human milk was more bifidogenic than the control formula and led to a microbiota profile similar to that for breast-fed infants.