Swarming motility: a multicellular behaviour conferring antimicrobial resistance
Swarming motility: a multicellular behaviour conferring antimicrobial resistance
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DOI：
10.1111/j.1462-2920.2008.01747.x
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发表时间：
2009-01-01
影响因子：
5.1
通讯作者：
Deziel, Eric
中科院分区：
文献类型：
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作者：
Lai, Sandra;Tremblay, Julien;Deziel, Eric
群集是一种社会运动，允许高度分化的细菌细胞迁移。群集与生物膜群落有许多相似之处，生物膜群落以其对抗菌剂的高抗性而闻名。我们在这里调查，如果群集行为也可能与广泛的抗菌素耐药表型。铜绿假单胞菌、大肠杆菌、粘质沙雷氏菌、泰国伯克霍尔德氏菌和枯草芽孢杆菌等5种菌群在13种抗生素的作用下，除抗菌肽外，对所有抗生素的耐药性均高于其它菌群。使用铜绿假单胞菌作为模型，这种多重耐药表型被证明是短暂的，并与群集状态内在相关。还观察到了菌群细胞对其他抗菌剂（如三氯生和重金属（亚砷酸盐））的耐药性。RND型外排泵（包括MexAB-OprM、MexCD-OprJ、MexEF-OprN和MexXY-OprM）和涉及周质葡聚糖的生物膜相关抗性机制似乎都不能解释群细胞的抗性。结合生物膜的高耐药性，这些结果支持了抗菌素耐药性是细菌多细胞性的一般特征的假设。因此，群集运动可能代表了一种社会行为形式，可用作研究生物膜抗生素耐药性的模型。
Swarming is a type of social motility allowing the migration of highly differentiated bacterial cells. Swarming shares many similarities with biofilm communities, which are notable for their high resistance to antimicrobial agents. We investigate here if the swarming behaviour could also be associated with a widespread antimicrobial resistant phenotype. Challenged with 13 antibiotics from various classes, swarm cells of Pseudomonas aeruginosa, Escherichia coli, Serratia marcescens, Burkholderia thailandensis and Bacillus subtilis showed higher resistance than their planktonic counterparts to all the antibiotics tested, except for the antimicrobial peptides. Using P. aeruginosa as a model, this multiresistant phenotype was shown to be transient and intrinsically linked to the swarming state. Resistance of swarm cells towards other antimicrobial agents, such as triclosan and a heavy metal (arsenite), was also observed. Neither RND-type efflux pumps, including MexAB-OprM, MexCD-OprJ, MexEF-OprN and MexXY-OprM, nor a biofilm-associated resistance mechanism involving periplasmic glucans, appear to account for the resistance of swarm cells. Together with the high resistance of biofilms, these results support the hypothesis that antimicrobial resistance is a general feature of bacterial multicellularity. Swarming motility might thus represent a form of social behaviour useful as a model to investigate biofilm antibiotic resistance.