Photodynamic activation of cholecystokinin 1 receptor is conserved in mammalian and avian pancreatic acini (代表作1)
Photodynamic activation of cholecystokinin 1 receptor is conserved in mammalian and avian pancreatic acini (代表作1)
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DOI：
10.3390/biomedicines11030885
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发表时间：
2023
期刊：
影响因子：
4.7
通讯作者：
Zong Jie Cui
中科院分区：
文献类型：
--
作者：
Jie Wang;Zong Jie Cui
Cholecystokinin 1 receptor (CCK1R) is the only G protein coupled receptor that is activated in type II photodynamic action, but whether this is a property common to both mammalian and avian species is not known. In this work, pancreatic acini were isolated from the rat, mouse, and Peking duck, and photodynamic CCK1R activation was examined. Isolated pancreatic acini were exposed to photosensitizer sulphonated aluminum phthalocyanine (SALPC) and photodynamic action elicited by a brief light-emitting diode (LED 675 nm) pulse (1.5 min); photodynamic CCK1R acti-vation was assessed by Fura-2 fluorescent calcium imaging. Photodynamic action was found to induce persistent calcium oscillations in rat, mouse, and Peking duck pancreatic acini, with the sensitivity order of mouse > rat > Peking duck. Photodynamically-activated CCK1R could be in-hibited reversibly by CCK1R antagonist devazepide (1 μM); photodynamic CCK1R activation was blocked by pre-incubation with 1O2 quencher Trolox C (300 µM). Sensitivity of photodynamic CCK1R activation was correlated with the increasing size of disordered region in intracellular loop 3. These data suggest that photodynamic CCK1R activation is conserved in both mammalian and avian species, as evidenced by the presence of the photodynamic activation motif “YFM” in transmembrane domain 3.
