Gene expression patterns in Euglena gracilis:: Insights into the cellular response to environmental stress
Gene expression patterns in Euglena gracilis:: Insights into the cellular response to environmental stress
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DOI：
10.1016/j.gene.2006.10.023
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发表时间：
2007-03-15
期刊：
影响因子：
3.5
通讯作者：
Levin, Mariano J.
中科院分区：
文献类型：
--
作者：
Ferreira, Veronica dos Santos;Rocchetta, Iara;Levin, Mariano J.
为了更好地了解纤细裸藻在不同胁迫条件(铬、链霉素和黑暗)下的基因表达情况，我们采用基因测序和微阵列分析的方法对纤细裸藻转录组进行了研究。首先，我们构建了细小E.gracilis UTEX株的非归一化cDNA文库，并对其进行了序列测定。这些EST中有610与植物或原生生物基因相似(e-Value；e(-10))。其次，通过将所有EST定位到镜片上，构建了微阵列。微阵列表达分析表明，在这610个EST中，有90个EST在不同的胁迫处理下发生了表达水平的变化(p<；0.05)。此外，我们检测到了10个EST，它们的表达水平发生了变化，与测试的压力无关。这些基因可能被认为是纤细乳杆菌中更大的一组与压力相关的基因的一部分。最后，我们根据这些可能的应激相关基因的表达谱确定了23个未知EST(U-EST)，表明它们可能与应激反应的细胞机制有关。(C)2006爱思唯尔B.V.保留所有权利。
To better understand Euglena gracilis gene expression under different stress conditions (Chromium, Streptomycin or darkness), we undertook a survey of the E. gracilis transcriptome by cDNA sequencing and microarray analysis. First, we constructed a non-normalized cDNA library from the E. gracilis UTEX strain and sequenced a total of 1000 cDNAs. Six hundred and ten of these ESTs were similar to either Plantae or Protistae genes (e-value < e(-10)). Second, microarrays were built by spotting all the EST's onto mirror slides. Microarray expression analysis indicated that 90 out of those 610 ESTs changed their expression level in response to different stress treatments (p < 0.05). In addition, we detected 10 ESTs that changed expression levels irrespective of the tested stress. These may be considered as part of a larger set of stress-related genes in E. gracilis. Finally, we identified 23 unknown ESTs (U-ESTs) following the expression profiles of these putative stress-related genes suggesting that they could be related to the cellular mechanism of stress response. (c) 2006 Elsevier B.V. All rights reserved.