Air and surface contamination patterns of meticillin-resistant Staphylococcus aureus on eight acute hospital wards
Air and surface contamination patterns of meticillin-resistant Staphylococcus aureus on eight acute hospital wards
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DOI：
10.1016/j.jhin.2013.12.005
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发表时间：
2014-03-01
影响因子：
6.9
通讯作者：
Humphreys, H.
中科院分区：
文献类型：
--
作者：
Creamer, E.;Shore, A. C.;Humphreys, H.
背景：耐甲氧西林金黄色葡萄球菌 (MRSA) 可以从医院空气和环境表面中回收。这构成了向患者传播的潜在风险。目的：在进行广泛的患者和环境采样时，调查从空气和环境表面回收的 MRSA 分离株与从患者身上回收的 MRSA 分离株之间的关联。方法：这是 2010 年和 2011 年期间对一家拥有 700 个床位的三级护理医院的 8 个病房的患者及其环境进行的前瞻性观察性研究。对所有病房进行了患者、空气和表面采样，并在病房进行了更广泛的环境采样高度依赖的海湾和一天中的特定时间。通过 DNA 微阵列分析和 spa 分型确定分离株的遗传相关性。结果：从 30/706 (4.3%) 患者和 19/132 (14.4%) 空气样本中回收到 MRSA。在 9/132 (6.8%) 的情况下，患者和空气样本均检测出 MRSA。在 32 个高度依赖的海湾中，从 12/161 (7.4%) 名患者、8/32 (25%) 份空气样本和 21/644 (3.3%) 份环境表面样本中检出 MRSA。在 10/132 (7.6%) 的情况下，在没有 MRSA 阳性患者的情况下，从空气中分离出 MRSA。患者人口统计数据与水疗分型和 DNA 微阵列分析相结合，揭示了四个可能的传播集群，其中患者和环境分离株被认为非常密切相关。结论：空气采样经常产生 MRSA，尤其是在高度依赖的海湾。环境和空气采样与患者人口统计数据、spa 分型和 DNA 微阵列分析相结合，表明存在其他情况下不明显的簇。 (C) 2014 年医疗保健感染协会。由爱思唯尔有限公司出版。保留所有权利。
Background: Meticillin-resistant Staphylococcus aureus (MRSA) can be recovered from hospital air and from environmental surfaces. This poses a potential risk of transmission to patients.Aim: To investigate associations between MRSA isolates recovered from air and environmental surfaces with those from patients when undertaking extensive patient and environmental sampling.Methods: This was a prospective observational study of patients and their environment in eight wards of a 700-bed tertiary care hospital during 2010 and 2011. Sampling of patients, air and surfaces was carried out on all ward bays, with more extended environmental sampling in ward high-dependency bays and at particular times of the day. The genetic relatedness of isolates was determined by DNA microarray profiling and spa typing.Findings: MRSA was recovered from 30/706 (4.3%) patients and from 19/132 (14.4%) air samples. On 9/132 (6.8%) occasions both patient and air samples yielded MRSA. In 32 high-dependency bays, MRSA was recovered from 12/161 (7.4%) patients, 8/32 (25%) air samples, and 21/644 (3.3%) environmental surface samples. On 10/132 (7.6%) occasions, MRSA was isolated from air in the absence of MRSA-positive patients. Patient demographic data combined with spa typing and DNA microarray profiling revealed four likely transmission clusters, where patient and environmental isolates were deemed to be very closely related.Conclusion: Air sampling yielded MRSA on frequent occasions, especially in high-dependency bays. Environmental and air sampling combined with patient demographic data, spa typing and DNA microarray profiling indicated the presence of clusters that were not otherwise apparent. (C) 2014 The Healthcare Infection Society. Published by Elsevier Ltd. All rights reserved.