Cytosolic overexpression of p62 sequestosome 1 in neoplastic prostate tissue
Cytosolic overexpression of p62 sequestosome 1 in neoplastic prostate tissue
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DOI：
10.1111/j.1365-2559.2005.02313.x
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发表时间：
2006-01-01
期刊：
影响因子：
6.4
通讯作者：
Sato, N
中科院分区：
文献类型：
--
作者：
Kitamura, H;Torigoe, T;Sato, N
目的：研究 p62 在各种前列腺组织中的表达，并证明 p62 在前列腺不同病理实体中表达的不同免疫组织化学模式。 p62 Sequestosome 1 (SQSTM1)基因产物是一种多功能蛋白，在正常成人组织中普遍表达。方法和结果：通过逆转录酶-聚合酶链式反应检测前列腺癌细胞系和组织中p62的过表达。对 73 例石蜡包埋的前列腺组织进行 p62 免疫组织化学染色。在九个正常和增生性前列腺标本中，p62 仅在前列腺上皮细胞核中呈阴性或弱阳性（模式 N），而大多数癌组织在细胞质中显示出强烈、均匀的 p62 染色（模式 C）。在前列腺癌样本中，91% 显示出阳性 C 型免疫反应性。在癌症周围患有高级别前列腺上皮内瘤变 (PIN) 的病例中，77% 呈 C 型。然而，在无前列腺癌 PIN 的患者标本中，只有 32% 的病例呈 C 型。 Western blot分析显示，所有癌细胞系在细胞质中均表达p62，但细胞核表达很少。结论：p62的胞质过表达是前列腺腺癌和高级别PIN中新的免疫组化特征，提示p62可能是前列腺恶性肿瘤的新标志物。
Aims: To investigate the expression of p62 in various prostatic tissues, and to demonstrate different immunohistochemical patterns of p62 expression in distinct pathological entities of the prostate. The p62 sequestosome 1 (SQSTM1) gene product is a multifunctional protein with ubiquitous expression in normal adult tissue.Methods and results: Overexpression of p62 was detected in prostate cancer cell lines and tissues by reverse transcriptase-polymerase chain reaction. Immunohistochemical staining for p62 was performed on 73 cases of paraffin-embedded prostatic tissue. p62 was negative or weakly positive only in the nucleus (pattern N) of prostatic gland epithelium in nine normal and hyperplastic prostate specimens, whilst most cancerous tissue showed intense, uniform staining for p62 in the cytoplasm (pattern C). Of the prostate cancer specimens, 91% showed positive pattern C immunoreactivity. Of the cases with high-grade prostatic intraepithelial neoplasia (PIN) around cancer, 77% showed pattern C. However, in specimens from the patients without prostate cancer PIN displayed pattern C in only 32% of cases. Western blot analysis showed that all cancer cell lines expressed p62 in the cytoplasm but there was little nuclear expression.Conclusion: Cytosolic overexpression of p62 is a novel immunohistochemical characteristic in prostatic adenocarcinoma and high-grade PIN, suggesting that p62 might be a novel marker for prostatic malignancy.