Application of immobilized Rhodobacter sphaeroides bacteria in hydrogen generation process under semi-continuous conditions
Application of immobilized Rhodobacter sphaeroides bacteria in hydrogen generation process under semi-continuous conditions
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DOI：
10.1016/j.ijhydene.2013.01.023
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发表时间：
2013-06
影响因子：
7.2
通讯作者：
R. Zagrodnik;M. Thiel;K. Seifert;M. Wlodarczak;M. Łaniecki
中科院分区：
文献类型：
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作者：
R. Zagrodnik;M. Thiel;K. Seifert;M. Wlodarczak;M. Łaniecki
以未改性和改性(AEAPTMS)VitraPOR®过滤多孔玻璃为载体，对球形红杆菌O.001细菌进行固定化。采用两种不同的玻璃，孔径分别为40-100m和100-160mμ。细菌生长采用Van Niel‘s培养基法，产氢反应采用改良的Biebl和Pfennig培养基法。本研究采用自行研制的平板光生物反应器(FPP)，在半连续条件下运行，处理能力为200cm3。得到的最大产氢速率为0.059 dm3H2/dm~3/h，最大产氢速率为4.2mLH2/mL苹果酸。系统相对稳定，因为每个系列持续约3个月。然后，产氢量下降。为了在多孔玻璃表面引入胺基，用AEAPTMS(3-(2-氨乙基)氨丙基)三甲氧基硅烷对其进行了改性。结果表明，改性和未改性的多孔玻璃都是稳定固定化微生物培养的合适材料。多孔玻璃基质具有较大的孔道，其活性和稳定性最好，而用胺对这些基质进行表面修饰可以增加固定化细胞的数量，但不能提高氢气的产率。
The non-modified and modified (AEAPTMS) VitraPOR®Filter porous glasses with thickness of 6.8 mm and diameter of 100 mm were applied as carriers for immobilization of Rhodobacter sphaeroides O.U. 001 bacteria. Two different glasses with pores of 40–100 and 100–160 μm were applied. The Van Niel's medium was applied for bacteria growth, whereas hydrogen generation reaction was performed with modified Biebl and Pfennig medium. Our own construction of Flat Plate Photobioreactor (FPP) with capacity of 200 cm3operating under semi-continuous conditions was applied in this study. The maximum hydrogen production rate obtained was 0.059 dm3H2/dm3/h, while the maximum hydrogen yield was 4.2 mol H2/mol malic acid. System was relatively stable because each series lasted about 3 months. Then the hydrogen production decreased. In order to introduce amine groups on the surface of porous glass, modification with AEAPTMS (3-(2-aminoethyl)aminopropyl)trimetoxysilane was performed. Obtained results proved that both modified and non-modified porous glasses are appropriate materials for stable immobilization of microbiological culture. The best activity and stability was achieved with porous glass matrix representing larger pores, whereas modification of the surface of these matrices with amine improved the amount of immobilized cells but did not improve the hydrogen yield.