Transcriptome profiling reveals genes involved in spine development during CsTTG1-regulated pathway in cucumber (Cucumis sativus L.)
Transcriptome profiling reveals genes involved in spine development during CsTTG1-regulated pathway in cucumber (Cucumis sativus L.)
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转录组分析揭示了黄瓜 (Cucumis sativus L.) 中 CsTTG1 调控途径中参与脊柱发育的基因
DOI：
10.1016/j.plantsci.2019.110354
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发表时间：
2020-02-01
期刊：
影响因子：
5.2
通讯作者：
Chen, Chunhua
中科院分区：
文献类型：
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作者：
Guo, Pei;Chang, Hualin;Chen, Chunhua
黄瓜（Cucumis sativus L.），一种肉质水果，覆盖着刺（多细胞毛状体），这对作物的经济价值有着至关重要的影响。以往的研究发现，CsTTG 1在黄瓜刺的起始和进一步分化中发挥重要作用，但CsTTG 1如何在分子水平上调控刺的形成仍知之甚少。在这项研究中，我们的特点是黄瓜35 S：CsTTG 1转基因T2系，OE-2，它承担相对较大和较长的刺相比，野生型（WT）的小和短的刺。表型测量和组织学分析表明，这种表型变化归因于细胞数量和大小的显着增加。通过DGE（Digital Gene Expression）分析比较OE-2和WT之间的卵巢表皮转录组，鉴定出1241个差异表达基因，其中712个基因在OE-2的卵巢表皮中显著上调，529个基因在OE-2的卵巢表皮中下调。XTH 23和Cyclin家族基因在OE-2中被显著激活，并且发现转录因子（TE）参与OE-2中的棘大小调节。进一步的分析证实GA参与了黄瓜果刺发育的调控。本研究为进一步解析黄瓜果刺调控的分子调控网络奠定了基础。
The cucumber (Cucumis sativus L.), a type of fleshy fruit, is covered with spines (multicellular trichomes), which have a crucial impact on the economic value of the crop. Previous studies have found that CsTTG1 plays important roles in the initiation and further differentiation of cucumber spines, but how spine formation is regulated at the molecular level by CsTTG1 remains poorly understood. In this study, we characterized a cucumber 35S:CsTTG1 transgenic T2 line, OE-2, which bears relatively large and long spines compared with the small and short spines of the wild type (WT). Phenotypic measurements and histological analyses revealed that this phenotypic change was attributed to significant increases in cell number and size. Comparison of ovary epidermis transcriptomes between OE-2 and WT by DGE (Digital Gene Expression) analysis identified 1241 differentially expressed genes, among which 712 genes were dramatically upregulated and 529 downregulated in the ovary epidermis of OE-2. XTH23 and Cyclin family genes were significantly activated in OE-2, and transcription factors (TEs) were found to participate in spine size regulation in OE-2. Further analyses confirmed that GA was implicated in the regulation of fruit spine development in cucumber. Thus, our study provides a foundation for dissecting the molecular regulatory networks of fruit spine control in cucumber.