p-Synephrine exhibits anti-adipogenic activity by activating the Akt/GSK3β signaling pathway in 3T3-L1 adipocytes
p-Synephrine exhibits anti-adipogenic activity by activating the Akt/GSK3β signaling pathway in 3T3-L1 adipocytes
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DOI：
10.1111/jfbc.13033
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发表时间：
2019-09-04
影响因子：
4
通讯作者：
Zheng, Xu-Xu
中科院分区：
文献类型：
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作者：
Guo, Li-Xia;Chen, Gang;Zheng, Xu-Xu
本研究旨在观察β-syneetrin对脂肪细胞分化的影响，并探讨其作用机制。我们发现，β-syneetrin通过降低CCAAT/增强子结合蛋白α（C/EBP α）和过氧化物酶体增殖物激活受体γ（PPAR γ）的表达水平，随后导致脂肪酸结合蛋白4（aP 2）表达降低，从而抑制3 T3-L1细胞脂肪形成。p-Synetrin处理显著激活蛋白激酶B（PKB/Akt）途径，并随后抑制糖原合成酶激酶3 β（GSK 3 β）活性。发现通过LiCl抑制GSK 3 β活性可部分改善上述效应。以上结果表明，β-syneetrin可能通过调节Akt信号通路和抑制脂肪生成相关蛋白而发挥抗脂肪生成作用。在世界各地的许多国家，包括西班牙塞维利亚和南非，柑橘经常被用作草药或膳食补充剂。在传统的中草药中，它被称为“枳实”、“枳实”或“止咳”，数百年来一直用于治疗各种消化问题。其主要的原生物碱，p-syneetamine，表现出脂解作用和能量消耗，它已迅速取代麻黄碱作为一个“麻黄免费”的替代膳食补充剂。本研究首次证实了β-syneetrin对3 T3-L1前脂肪细胞成脂的抑制作用，其机制可能是通过调节Akt信号通路，进而抑制成脂相关蛋白的表达。本研究可为β-辛烯酮的减肥/降脂作用机理提供理论依据，也可为膳食补充剂的实际应用提供科学的实验依据。β-Synetamine可用于预防和治疗代谢综合征。
This work aimed to investigate the effects of p-synephrine on the differentiation of adipocyte and explore the underlying mechanism. We found that p-synephrine suppressed the 3T3-L1 cell adipogenesis by reducing the expression level of CCAAT/enhancer-binding protein alpha (C/EBP alpha) and peroxisome proliferator-activated receptor gamma (PPAR gamma), which subsequently led to a reduction in the fatty acid-binding protein 4 (aP2) expression. p-Synephrine treatment markedly activated the protein kinase B (PKB/Akt) pathway and sequentially inhibited glycogen synthase kinase 3 beta (GSK3 beta) activity. Inhibition of GSK3 beta activity by LiCl was found to partially ameliorate the above-mentioned effects. All these data suggested that p-synephrine exhibited the anti-adipogenic effects via the regulation of Akt signaling pathway and the suppression of adipogenesis-related proteins. Practical applications Citrus aurantium often uses as herbal or dietary supplement in various countries around the world, including in Seville, Spain and South Africa. In traditional Chinese herbs, it is referred to as "Fructus aurantii immaturus," "Zhi shi," or "Zhi ke," and has been used for hundreds of years for various digestive problems. Its primary protoalkaloid, p-synephrine, exhibited lipolytic effects and energy expenditure, which has rapidly replaced ephedrine as an "ephedra-free" alternative dietary supplement. The current study firstly demonstrated the anti-adipogenic effects of p-synephrine in 3T3-L1 preadipocytes, which was due to the regulation of Akt signaling pathway and the subsequent suppression of adipogenesis-related proteins. The present study may offer invaluable opinions into the mechanisms of body weight/fat-losing activities of p-synephrine in theory, and scientific experimental evidence on dietary supplement in practice. p-Synephrine could be utilized for the preventive and therapeutic uses against metabolic syndrome.