Critical roles of interferon regulatory factor 4 in CD11bhighCD8α- dendritic cell development
Critical roles of interferon regulatory factor 4 in CD11bhighCD8α- dendritic cell development
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DOI：
10.1073/pnas.0402139101
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发表时间：
2004-06-15
影响因子：
11.1
通讯作者：
Kumatori, A
中科院分区：
文献类型：
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作者：
Suzuki, S;Honma, K;Kumatori, A
干扰素调节因子（IRFs）是一个转录因子家族，在免疫系统的稳态和功能中发挥重要作用。最近的研究表明，IRF-8对于CD 11b（低）CD 8 alpha（+）常规树突状细胞（DC）和浆细胞样DC的发育至关重要。在这里，我们表明IRF-4是重要的CD 11b（高）CD 8 α（-）的传统的DC。在添加GM-CSF或Flt 3配体的两种培养体系中，IRF-4（-/-）小鼠骨髓中CD 11b（高）DC的发育严重受损。在IRF-4（-/-）脾脏中，CD 4（+）CD 8 α（-）DC（CD 11b（高）DC的主要亚群）数量严重减少。IRF-4和IRF-8分别在大多数CD 11b（高）CD 4（+）CD 8 α（-）DC和CD 11b（低）CD 8 α（+）DC中以相互排斥的方式表达。这些结果意味着IRF-4和IRF-8选择性地在表达它们的DC亚群的发育中发挥关键作用。
IFN regulatory factors (IRFs) are a family of transcription factors that play an essential role in the homeostasis and function of immune systems. Recent studies indicated that IRF-8 is critical for the development of CD11b(low)CD8alpha(+) conventional dendritic cells (DCs) and plasmacytoid DCs. Here we show that IRF-4 is important for CD11b(high)CD8alpha(-) conventional DCs. The development of CD11b(high) DCs from bone marrow of IRF-4(-/-) mice was severely impaired in two culture systems supplemented with either GM-CSF or Flt3-ligand. In the IRF-4(-/-) spleen, the number of CD4(+)CD8alpha(-) DCs, a major subset of CD11b(high) DCs, was severely reduced. IRF-4 and IRF-8 were expressed in the majority of CD11b(high) CD4(+)CD8alpha(-) DCs and CD11b(low)CD8alpha(+) DCs, respectively, in a mutually exclusive manner. These results imply that IRF-4 and IRF-8 selectively play critical roles in the development of the DC subsets that express them.