Plasma 99th percentile reference limits for cardiac troponin and creatine kinase MB mass for use with European Society of Cardiology American College of Cardiology consensus recommendations
Plasma 99th percentile reference limits for cardiac troponin and creatine kinase MB mass for use with European Society of Cardiology American College of Cardiology consensus recommendations
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DOI：
10.1373/49.8.1331
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发表时间：
2003-08-01
影响因子：
9.3
通讯作者：
Murakami, MM
中科院分区：
文献类型：
--
作者：
Apple, FS;Quist, HE;Murakami, MM
背景资料：欧洲心脏病学会/美国心脏病学会（ESC/ACC）关于心肌梗死（MI）定义的共识文件是基于心肌肌钙蛋白或肌酸激酶（CK）MB质量增加超过第99百分位数参考限值。本研究的目的是确定血浆（肝素）的第99百分位数的参考限值领先的体外诊断心肌肌钙蛋白和CKMB质量assays.Methods：血液（肝素血浆）是从健康成人（n = 696;年龄范围，18-84岁）分层的性别和种族。通过8种测定方法测定心肌肌钙蛋白I（cTnI）和T（cTnT）以及CKMB质量浓度。通过非参数统计分析确定参考限。结果：两种cTnI测定法显示至少1.2- 2.5倍高99。男性与女性相比，百分位数，男性的平均浓度显著高于女性（P < 0.05）。两项cTnI检测还显示，黑人的第99百分位数比白人高1.1- 2.8倍，黑人的平均浓度显著更高（P = 0.05）。在最低测量的第99百分位数（0.06 mug/L）和最高测量的第99百分位数（0.8 mug/L）之间存在13倍的方差。所有CKMB检测均显示男性第99百分位数比女性高1.2至2.6倍，男性的平均浓度显著高于女性（P
Background: The European Society of Cardiology/ American College of Cardiology (ESC/ACC) consensus document for definition of myocardial infarction (MI) is predicated on increased cardiac troponin or creatine kinase (CK) MB mass above the 99th percentile reference limit. The purpose of this study was to determine the plasma (heparin) 99th percentile reference limits for the leading in vitro diagnostic cardiac troponin and CKMB mass assays.Methods: Blood (heparin plasma) was obtained from healthy adults (n = 696; age range, 18-84 years) stratified by gender and ethnicity. Cardiac troponin I (cTnI) and T (cTnT) and CKMB mass concentrations were measured by eight assays. Reference limits were determined by nonparametric statistical Analysis.Results: Two cTnI assays demonstrated at least a 1.2- to 2.5-fold higher 99th. percentile for males vs females, with the mean concentrations significantly higher for males (P < 0.05). Two cTnI assays also demonstrated a 1.1- to 2.8-fold higher 99th percentile for blacks vs Caucasians, with the mean concentrations significantly higher for blacks (P = 0.05). There Was a 13-fold variance between the lowest measured 99th percentile (0.06 mug/L) and the highest (0.8 mug/L). All CKMB assays demonstrated a 1.2 to 2.6-fold higher 99th percentile for males vs females, with mean concentrations significantly higher for males (P