Changes in the secretion of inhibin and steroid hormones during induced follicular atresia after hypophysectomy in the rat.
Changes in the secretion of inhibin and steroid hormones during induced follicular atresia after hypophysectomy in the rat.
复制标题
大鼠垂体切除术后诱导卵泡闭锁过程中抑制素和类固醇激素分泌的变化。
DOI：
10.1016/0024-3205(87)90701-6
复制
发表时间：
1987
期刊：
影响因子：
6.1
通讯作者：
S. Sasamoto
中科院分区：
文献类型：
--
作者：
H. Kaneko;K. Taya;S. Sasamoto
在发情前期1100小时，对卵泡闭锁期间窦卵泡功能的顺序变化进行了研究。在Hypox后6小时内，卵巢静脉血浆（OVP）中孕酮（P）、睾酮（T）和雌二醇-17 β（E）浓度突然下降，卵泡排卵能力下降。Hypox后6小时，在所有动物中仍由人绒毛膜促性腺激素（hCG）诱导排卵，但与发情前期当天1100小时给予hCG的组相比，卵母细胞数量显著减少。Hypox后9小时，所有大卵泡（直径> 400μm）的几个颗粒细胞均表现出闭锁的形态学迹象。Hypox后12 h，所有大中型卵泡（直径200-400 μm）均出现晚期闭锁，几乎所有卵泡对hCG无排卵反应。OVP中抑制素活性的下降速度比类固醇激素的下降速度慢，并且在手术后18小时仍保持53%的初始抑制素活性。抑制素活性在24小时进一步降低至初始水平的7%，并且在Hypox后48小时检测不到。这些结果表明，完全发育的Graafian卵泡逐渐失去分泌类固醇激素的能力，而在闭锁过程中类固醇激素的分泌迅速减少。
Sequential changes in the function of antral follicles during the period of follicular atresia were investigated after hypohysectomy (Hypox) at 1100 hr on proestrus. Within 6 hours after Hypox, concentrations of progesterone (P), testosterone (T) and estradiol-17β (E) decreased abruptly in ovarian venous plasma (OVP) and follicles showed a reduced ability to ovulate. Six hours after Hypox, ovulation was still induced by human chorionic gonadotropin (hCG) in all animals but with significantly fewer number of oocytes compared to the group given hCG at 1100 hr on the day of proestrus. Nine hours after Hypox, several granulosa cells of all large follicles (> 400μm in diameter) exhibited morphological signs of atresia. Twelve hours after Hypox, all large and medium sized (200–400 μm in diameter) follicles showed advanced stages of atresia and almost all follicles failed to ovulate in response to hCG. Inhibin activity in OVP declined more slowly compared to the profiles of steroid hormones and 53% of the initial inhibin activity was still maintained at 18 hours after the operation. Inhibin activity further decreased to 7% of the initial level at 24 hours and was undetectable by 48 hours after Hypox. These results suggest that fully developed Graafian follicles gradually lose their ability to secrete inhibin in contrast to the rapid decrease in secretion of steroid hormone during the process of atresia.