Zebrafish as a new model for rhododendrol-induced leukoderma.
Zebrafish as a new model for rhododendrol-induced leukoderma.
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斑马鱼作为杜鹃醇诱导的白皮病的新模型。
DOI：
10.1111/pcmr.13005
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发表时间：
2021
期刊：
影响因子：
--
通讯作者：
Hamamoto A.
中科院分区：
文献类型：
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作者：
Hayazaki M;Hatano O;Shimabayashi S;Akiyama T;Takemori H;Hamamoto A.
特发性白斑病是一种皮肤病，其特征是由于黑素细胞功能障碍或缺乏而导致的皮肤色素斑片状丧失。Rhododendrol（RD）于2008年在日本被批准为化妆品成分。然而，它被证明在大约20，000名客户中诱导白细胞增多症。预测细胞毒性，特别是体内黑素细胞的细胞毒性，以避免这种不利影响。由于禁止使用高等脊椎动物进行药物和毒理学试验，因此我们使用了斑马鱼，其黑素细胞受到与哺乳动物相似的机制的调节。斑马鱼幼虫在养殖水中用RD处理3天，这导致身体变亮，伴随着黑色素细胞数量的减少。有趣的是，在培养皿的底部发现了黑色颗粒，这表明黑色素细胞从体内脱落。此外，RT-PCR分析表明，黑素细胞特异性基因的mRNA水平显着较低。RD处理的幼虫活性氧的产生增加。用其他酚类化合物处理鱼，据报道会引起白细胞增多症，也会引起色素脱失和黑色素细胞损失。这些结果表明，斑马鱼幼虫可用于评估由化学品引起的白血病，包括RD。
Idiopathic leukoderma is a skin disorder characterized by patchy loss of skin pigmentation due to melanocyte dysfunction or deficiency. Rhododendrol (RD) was approved as a cosmetic ingredient in Japan in 2008. However, it was shown to induce leukoderma in approximately 20,000 customers. The prediction of cytotoxicity, especially to melanocytes in vivo, is required to avoid such adverse effects. Since the use of higher vertebrates is prohibited for medicinal and toxicological assays, we used zebrafish, whose melanocytes were regulated by mechanisms similar to mammals. Zebrafish larvae were treated with RD in breeding water for 3 days, which caused body lightening accompanied by a decrease in the number of melanophores. Interestingly, black particles were found at the bottom of culture dishes, suggesting that the melanophores peeled off from the body. In addition, RT‐PCR analysis suggested that the mRNA levels of melanophore‐specific genes were significantly low. An increase in the production of reactive oxygen species was found in larvae treated with RD. The treatments of the fish with other phenol compounds, which have been reported to cause leukoderma, also induced depigmentation and melanophore loss. These results suggest that zebrafish larvae could be used for the evaluation of leukoderma caused by chemicals, including RD.