Effect of culture environment on gene expression and developmental characteristics in IVF-derived embryos
Effect of culture environment on gene expression and developmental characteristics in IVF-derived embryos
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DOI：
10.1016/j.theriogenology.2005.09.028
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发表时间：
2006-01-07
期刊：
影响因子：
2.8
通讯作者：
Evans, ACO
中科院分区：
文献类型：
--
作者：
Lonergan, P;Fair, T;Evans, ACO
人们普遍认为，哺乳动物着床前胚胎对环境非常敏感，培养条件会影响胚胎在出生前和出生后的生长和发育潜力。有证据表明，虽然牛体外胚胎生产过程中的培养条件对早期胚胎的发育潜力有一定影响，但卵母细胞的内在质量是决定卵母细胞发育到囊胚阶段比例的关键因素。此外，有证据表明，从低温耐受性、基因表达模式和受孕能力等方面评价，受精后胚胎培养时期是影响囊胚质量的最关键时期。本文以牛为研究对象，综述了受精后培养环境对胚胎基因表达和质量影响的相关文献。(c) 2005爱思唯尔公司版权所有。
It is generally accepted that mammalian preimplantation embryos are sensitive to their environment and that conditions of culture can affect future growth and developmental potential both pre- and postnatally. Evidence suggests that while culture conditions during bovine in vitro embryo production can impact somewhat on the developmental potential of the early embryo, the intrinsic quality of the oocyte is the key factor determining the proportion of oocytes developing to the blastocyst stage. In addition, evidence suggests that the period of post fertilization embryo culture is the most critical period affecting blastocyst quality assessed in terms of cryotolerance, gene expression pattern and ability to establish a pregnancy. This paper reviews the current literature, with emphasis on the bovine model, demonstrating evidence for an effect of post fertilization culture environment on embryo gene expression and quality. (c) 2005 Elsevier Inc. All rights reserved.