Differential expression of sodium channels in acutely isolated myelinating and non-myelinating Schwann cells of rabbits.
Differential expression of sodium channels in acutely isolated myelinating and non-myelinating Schwann cells of rabbits.
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急性分离的兔髓鞘和非髓鞘雪旺细胞中钠通道的差异表达。
DOI：
10.1113/jphysiol.1993.sp019871
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发表时间：
1993
期刊：
影响因子：
--
通讯作者：
Chiu,SY
中科院分区：
文献类型：
--
作者：
Chiu,SY
1.单通道记录由从雪旺细胞的细胞体区域切除的外向斑中的电压敏感性钠通道制成。从10周龄家兔的坐骨神经中新鲜分离细胞，并目视识别髓鞘形成和非髓鞘形成施旺细胞以进行记录。2.在约75%的从非髓鞘形成雪旺细胞的细胞体获得的贴片（n = 41）中观察到钠通道活性。与此相反，钠通道中没有观察到的补丁（n = 78）从髓鞘化雪旺细胞的细胞体。3.横断坐骨神经并在记录前使沃勒变性在体内发生5 - 11天，导致从髓鞘形成细胞的细胞体获得的约30%的斑块（n = 33）中出现钠通道活性。4.有趣的是，虽然免疫细胞化学和石房蛤毒素结合实验表明，明确存在的钠通道在髓鞘形成的许旺细胞的质膜（无论是在细胞体和在paranodal区域），本研究表明，这些通道是不存在的正常髓鞘形成的许旺细胞的索马区域。
1. Single channel recordings were made of voltage‐sensitive sodium channels in outside‐out patches excised from the cell body region of Schwann cells. The cells were freshly isolated from sciatic nerves of 10‐week‐old rabbits and both myelinating and non‐myelinating Schwann cells were visually identified for recordings. 2. Sodium channel activities were observed in approximately 75% of patches (n = 41) obtained from cell bodies of non‐myelinating Schwann cells. In contrast, sodium channels were not observed in patches (n = 78) obtained from cell bodies of myelinating Schwann cells. 3. Transection of the sciatic nerves and allowing Wallerian degeneration to occur for 5‐11 days in vivo prior to recording resulted in the appearance of sodium channel activities in approximately 30% of the patches (n = 33) obtained from the cell body of the myelinating cells. 4. It is interesting that although immunocytochemical and saxitoxin binding experiments indicate the clear presence of sodium channels in the plasmalemma of myelinating Schwann cells (both at the cell body and at the paranodal region), the present study suggests that these channels are absent from the soma region of normal myelinating Schwann cells.