Expression of RNA-binding protein Musashi in hair follicle development and hair cycle progression.
Expression of RNA-binding protein Musashi in hair follicle development and hair cycle progression.
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DOI：
10.2353/ajpath.2006.050469
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发表时间：
2006
期刊：
影响因子：
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通讯作者：
Y. Sugiyama-Nakagiri;M. Akiyama;S. Shibata;H. Okano;H. Shimizu
中科院分区：
文献类型：
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作者：
Y. Sugiyama-Nakagiri;M. Akiyama;S. Shibata;H. Okano;H. Shimizu
上皮干细胞位于毛囊（HF）隆起区域，具有分化为各种皮肤上皮细胞的能力。进化上保守的Musashi RNA结合蛋白家族与神经祖细胞的维持和/或不对称细胞分裂相关，并且哺乳动物Musashi蛋白在各种上皮干/祖细胞中表达，包括肠、胃和乳腺。因此，我们推测Musashi可能在HF上皮的干细胞和早期祖细胞中表达。逆转录-聚合酶链反应和免疫印迹法在培养的小鼠角质形成细胞中鉴定出Musashi-1（Msi-1）和Musashi-2（Msi-2）mRNA和蛋白，但在人角质形成细胞中仅鉴定出Msi-1。在小鼠中，免疫组织化学研究表明，Msi-1和Msi-2表达在表皮和HFs从E14.5直到成年。在生长期早期，Msi-1和Msi-2在隆突细胞和次级生殖细胞中表达，随后在生长期晚期在内根鞘（IRS）细胞中表达，尤其是中间IRS细胞。在人类皮肤中，在胎儿HF细胞中检测到Msi-1，但在成人HF中未检测到。这些观察结果表明，武藏功能不仅在早期祖细胞的不对称分裂，但也在IRS细胞的分化在HF发展和头发周期的进展。
Epithelial stem cells reside in the hair follicle (HF) bulge region and possess the ability to differentiate into a variety of cutaneous epithelial cells. The evolutionarily conserved Musashi family of RNA-binding proteins is associated with maintenance and/or asymmetric cell division of neural progenitor cells, and a mammalian Musashi protein is expressed in various epithelial stem/progenitor cells, including gut, stomach, and mammary gland. Thus, we hypothesized that Musashi might be expressed in stem cells and early progenitor cells of HF epithelium. Reverse transcriptase-polymerase chain reaction and immunoblotting identified Musashi-1 (Msi-1) and Musashi-2 (Msi-2) mRNA and protein in cultured mouse keratinocytes, but only Msi-1 was identified in human keratinocytes. In mice, immunohistochemical studies showed that Msi-1 and Msi-2 were expressed in the epidermis and HFs from E14.5 until adulthood. In the early anagen phase, Msi-1 and Msi-2 were expressed in the bulge and secondary germ cells and subsequently in inner root sheath (IRS) cells, especially the middle IRS cells, during the late anagen phase. In human skin, Msi-1 was detected in fetal HF cells but not in adult HFs. These observations suggest that Musashi functions not only in the asymmetric division of early progenitor cells but also in the differentiation of IRS cells during HF development and hair cycle progression.