An immune-based tool platform for in vivo cell clearance.
An immune-based tool platform for in vivo cell clearance.
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DOI：
10.26508/lsa.202201869
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发表时间：
2023-08
影响因子：
4.4
通讯作者：
中科院分区：
文献类型：
--
作者：
一个工具平台，允许探索免疫介导的细胞毒性疗法，以靶向癌症以外疾病的病理细胞。病理细胞的免疫靶向在肿瘤学中取得了成功，并正在扩展到其他病理生物学背景。在这里，我们提出了一个灵活的平台，允许用表面表达的模型抗原卵清蛋白（OVA）标记感兴趣的细胞，其可以通过抗原特异性T细胞或新开发的OVA抗体消除。我们证明，肝细胞可以有效地通过任何方式的目标。相比之下，与肺纤维化相关的促纤维化成纤维细胞仅在初始实验中被T细胞消除，这减少了纤维化模型中的胶原沉积。这个新的实验平台将促进基于免疫的方法的发展，以清除体内潜在的病理细胞类型。
A tool platform that allows the exploration of immune-mediated cytotoxic therapies to target pathological cells in diseases beyond cancer. Immunological targeting of pathological cells has been successful in oncology and is expanding to other pathobiological contexts. Here, we present a flexible platform that allows labeling cells of interest with the surface-expressed model antigen ovalbumin (OVA), which can be eliminated via either antigen-specific T cells or newly developed OVA antibodies. We demonstrate that hepatocytes can be effectively targeted by either modality. In contrast, pro-fibrotic fibroblasts associated with pulmonary fibrosis are only eliminated by T cells in initial experiments, which reduced collagen deposition in a fibrosis model. This new experimental platform will facilitate development of immune-based approaches to clear potential pathological cell types in vivo.
影响因子：
14.9
作者：
Sandhu U;Cebula M;Behme S;Riemer P;Wodarczyk C;Metzger D;Reimann J;Schirmbeck R;Hauser H;Wirth D
通讯作者：
Wirth D