A single locus determines sensitivity to bacterial flagellin in Arabidopsis thaliana
A single locus determines sensitivity to bacterial flagellin in Arabidopsis thaliana
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DOI：
10.1046/j.1365-313x.1999.00451.x
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发表时间：
1999-05-01
期刊：
影响因子：
7.2
通讯作者：
Boller, T
中科院分区：
文献类型：
--
作者：
Gómez-Gómez, L;Felix, G;Boller, T
与真细菌鞭毛蛋白最保守结构域相对应的多肽在不同植物种类的细胞中起着强有力的激发子作用。在完整的拟南芥幼苗中，这些肽（flg22和flg15）引起胼胝质沉积，诱导编码致病相关蛋白的基因，并强烈抑制生长。一半最大生长抑制发生在肽浓度约100 nM。相比之下，代表植物相关细菌A. tumefaciens和R. meliloti相应鞭毛结构域的肽即使在10 μ m浓度下也没有活性。除了Ws-0外，所有被测试的生态型A. thaliana都对flg22敏感。Ws-0分别与敏感生态型Col-0和La-er杂交，产生敏感的F1幼苗。在两个杂交组合的F2代中，敏感性分离为单个性状，5号染色体标记，比例为3:1。被称为FLS-1的感知鞭毛蛋白位点的优势表明，它编码了一种对鞭毛蛋白信号感知很重要的元件。
Peptides corresponding to the most conserved domain of eubacterial flagellin act as potent elicitors in cells of different plant species. In intact Arabidposis thaliana seedlings these peptides (flg22 and flg15) caused callose deposition, induction of genes coding for pathogenesis-related proteins and a strong inhibition of growth. Half-maximal growth inhibition occurred at peptide concentrations of approximately 100 nM. In contrast, peptides representing the corresponding flagellin domains of the plant-associated bacteria A. tumefaciens and R. meliloti were inactive even at concentrations of 10 mu M. With the exception of Ws-0, all ecotypes of A. thaliana tested were sensitive to flg22. Crosses of Ws-0 with the sensitive ecotypes Col-0 and La-er, respectively, resulted in sensitive F1 seedlings. In the F2 generation of both crosses, sensitivity segregated as a single trait with markers of chromosome 5 and a ratio of 3:1. Dominance of the locus sensing flagellin, termed FLS-1, suggests that it encodes an element which is important for the perception of the flagellin signal.