Integrated microfluidics for chromosome engineering preparation, transportation and manipulation
Integrated microfluidics for chromosome engineering preparation, transportation and manipulation
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DOI：
10.1679/aohc.65.465
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发表时间：
2002-12-01
影响因子：
--
通讯作者：
Yokoyama, H
中科院分区：
文献类型：
--
作者：
Inoue, T;Takahashi, K;Yokoyama, H
介绍了用于染色体运输和操作的微流体系统的设计和制作过程。利用各向异性湿法腐蚀技术在单晶硅衬底上制备出流体网络微通道。用机械泵将含有染色体的缓冲液注入通道后，进行染色体电泳，以实现染色体的精确运输。染色体放置在直流（DC）和交流（AC）电场的行为进行了详细解释。在这项研究中观察到的频率和大小依赖的振幅的染色体显示存在的惯性流体动力学效应的振荡运动。利用旋转交流电场，染色体表面电荷的诱导极化和双电层的作用，使染色体发生旋转。因此，这项研究显示了在微芯片上进行染色体工程的可能性，有望开发出用于染色体研究的新设备。
The design and fabrication process of micro-fluidics for chromosome transportation and manipulation are described. Micro-channels for fluid networks could be built on single-crystal silicon substrates by anisotropic wet etching. After injection of a chromosome-containing buffer solution into the channels by a mechanical pump, chromosome electrophoresis was carried out for its precise transportation. The behavior of chromosomes placed in direct (DC) and alternating (AC) electric fields is explained in detail. The frequency and size dependent amplitude of the chromosome observed in this study showed the existence of inertial hydrodynamic effects in an oscillatory motion. Using a rotating AC electric field, chromosomes could be rotated due to the induced polarization of surface charge and electric double layer of the chromosomes. This study thus shows the possibility of chromosome engineering on microchips, which is expected to enable the development of new devices for chromosome research.