Interleukin 6 promotes vasculogenesis of murine brain microvessel endothelial cells
Interleukin 6 promotes vasculogenesis of murine brain microvessel endothelial cells
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DOI：
10.1006/cyto.1999.0599
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发表时间：
2000-06-01
期刊：
影响因子：
3.8
通讯作者：
Fabry, Z
中科院分区：
文献类型：
--
作者：
Fee, D;Grzybicki, D;Fabry, Z
白细胞介素6（IL-6）是一种细胞因子，作用于广泛的组织，影响细胞生长和分化。在这里，我们表明，IL-6在中枢神经系统（CNS）的早期血管发育（血管发生）中发挥作用。我们报道了IL-6在体外诱导脑微血管内皮细胞增殖。此外，IL-6显著加速这些细胞在Matrigel基底基质中形成管状结构。此外，IL-6 mRNA在两种生理条件下体内表达，其中CNS中的血管化是重要的：（1）在正常脑发育期间，（2）在创伤性脑损伤的愈合过程期间。IL-6 mRNA的表达与血管内皮生长因子（VEGF）mRNA在发育中的表达一致，出生后表达下降。然而，IL-6的mRNA可以检测到愈合的成年小鼠脑组织中的原位杂交与强烈的组织重组期相一致。IL-6 mRNA在正常脑发育期间和脑损伤部位的瞬时上调以及IL-6对体外血管发生的作用表明IL-6可能在CNS中血管形成的正常生理学中起作用，（C）2000 Academic Press.
Interleukin 6 (IL-6) is a cytokine that acts on a wide range of tissues influencing cell growth and differentiation. Here we show that IL-6 plays a role in the early vascular development (vasculogenesis) in the central nervous system (CNS). We report that IL-6 induces the proliferation of brain microvascular endothelial cells in vitro. Furthermore, IL-6 significantly accelerates the formation of tube-like structures by these cells in Matrigel basement matrix. Moreover, IL-6 mRNA is expressed in vivo in two physiological conditions in which vascularization in the CNS is important: (1) during normal brain development, (2) during the healing process of a traumatic brain injury. Expression of IL-6 mRNA coincides with the expression of vascular endothelial growth factor (VEGF) mRNA in the developing brain with decreasing expression following birth. However, IL-6 mRNA can be detected in the healing adult murine brain tissue by in situ hybridization coinciding with the period of intense tissue reorganization. The transient upregulation of IL-6 mRNA during normal brain development and at brain injury site and the effect of IL-6 on in vitro vasculogenesis suggest that IL-6 may play a role in normal physiology of vascularization in the CNS, (C) 2000 Academic Press.